| Autor: |
Wang HY; Department of Entomology, Nanjing Agricultural University; Key Laboratory of Integrated Pest Management of Crop of East China, Ministry of Agriculture., Yu ZQ; Department of Entomology, Nanjing Agricultural University; Key Laboratory of Integrated Pest Management of Crop of East China, Ministry of Agriculture., Ren XY; Department of Entomology, Nanjing Agricultural University; Key Laboratory of Integrated Pest Management of Crop of East China, Ministry of Agriculture., Li YX; Department of Entomology, Nanjing Agricultural University; Key Laboratory of Integrated Pest Management of Crop of East China, Ministry of Agriculture., Yan ZC; Department of Entomology, Nanjing Agricultural University; Key Laboratory of Integrated Pest Management of Crop of East China, Ministry of Agriculture; Sanya Institute of Nanjing Agricultural University; zcyan@njau.edu.cn. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of visualized experiments : JoVE [J Vis Exp] 2023 Oct 06 (200). Date of Electronic Publication: 2023 Oct 06. |
| DOI: |
10.3791/66032 |
| Abstrakt: |
Parasitoid wasps are a diverse group of hymenopteran insects that serve as invaluable resources for pest biocontrol. To ensure successful parasitism, parasitoid wasps inject venom into their hosts to suppress their hosts' immunity, modulate hosts' development, metabolism, and even behavior. With over 600,000 estimated species, the diversity of parasitoid wasps surpasses that of other venomous animals, such as snakes, cone snails, and spiders. Parasitoid wasp venom is an underexplored source of bioactive molecules with potential applications in pest control and medicine. However, collecting parasitoid venom is challenging due to the inability to use direct or electrical stimulation and the difficulty in dissection because of their small size. Trichogramma is a genus of tiny (~0.5 mm) egg parasitoid wasps that are widely used for the biological control of lepidopteran pests in both agriculture and forests. Here, we report a method for extracting venom from T. dendrolimi using artificial hosts. These artificial hosts are created with polyethylene film and amino acid solutions and then inoculated with Trichogramma wasps for parasitism. The venom was subsequently collected and concentrated. This method enables the extraction of large amounts of Trichogramma venom while avoiding contamination from other tissues caused by dissection, a common issue in venom reservoir dissection protocols. This innovative approach facilitates the study of Trichogramma venom, paving the way for new research and potential applications. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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