FLight Biofabrication Supports Maturation of Articular Cartilage with Anisotropic Properties.

Autor: Puiggalí-Jou A; Tissue Engineering + Biofabrication Laboratory, Department of Health Sciences & Technology, ETH Zürich, Otto-Stern-Weg 7, Zürich, 8093, Switzerland., Rizzo R; Tissue Engineering + Biofabrication Laboratory, Department of Health Sciences & Technology, ETH Zürich, Otto-Stern-Weg 7, Zürich, 8093, Switzerland.; John A. Paulson School of Engineering and Applied Sciences, Harvard University, 52 Oxford Street, Cambridge, MA, 02138, USA.; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA., Bonato A; Tissue Engineering + Biofabrication Laboratory, Department of Health Sciences & Technology, ETH Zürich, Otto-Stern-Weg 7, Zürich, 8093, Switzerland., Fisch P; Tissue Engineering + Biofabrication Laboratory, Department of Health Sciences & Technology, ETH Zürich, Otto-Stern-Weg 7, Zürich, 8093, Switzerland., Ponta S; Tissue Engineering + Biofabrication Laboratory, Department of Health Sciences & Technology, ETH Zürich, Otto-Stern-Weg 7, Zürich, 8093, Switzerland., Weber DM; Division of Hand Surgery, University Children's Hospital Zürich, University of Zürich, Zürich, 8032, Switzerland., Zenobi-Wong M; Tissue Engineering + Biofabrication Laboratory, Department of Health Sciences & Technology, ETH Zürich, Otto-Stern-Weg 7, Zürich, 8093, Switzerland.
Jazyk: angličtina
Zdroj: Advanced healthcare materials [Adv Healthc Mater] 2024 May; Vol. 13 (12), pp. e2302179. Date of Electronic Publication: 2023 Nov 12.
DOI: 10.1002/adhm.202302179
Abstrakt: Tissue engineering approaches that recapitulate cartilage biomechanical properties are emerging as promising methods to restore the function of injured or degenerated tissue. However, despite significant progress in this research area, the generation of engineered cartilage constructs akin to native counterparts still represents an unmet challenge. In particular, the inability to accurately reproduce cartilage zonal architecture with different collagen fibril orientations is a significant limitation. The arrangement of the extracellular matrix (ECM) plays a fundamental role in determining the mechanical and biological functions of the tissue. In this study, it is shown that a novel light-based approach, Filamented Light (FLight) biofabrication, can be used to generate highly porous, 3D cell-instructive anisotropic constructs that lead to directional collagen deposition. Using a photoclick-based photoresin optimized for cartilage tissue engineering, a significantly improved maturation of the cartilaginous tissues with zonal architecture and remarkable native-like mechanical properties is demonstrated.
(© 2023 The Authors. Advanced Healthcare Materials published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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