Molecular and morphometric study of Brazilian populations of Psychodopygus davisi.

Autor:	Rodrigues BL; Programa de Pós-Graduação em Saúde Pública, Faculdade de Saúde Pública da Universidade de São Paulo (FSP/USP), São Paulo, Brazil., da Silva Costa G; Programa de Pós-Graduação em Biologia Experimental, Fundação Universidade Federal de Rondônia, Porto Velho, Brazil.; Laboratório Central de Saúde Pública do Estado de Rondônia, LACEN-RO, Porto Velho, Brazil., Godoy RE, Pereira Júnior AM; Laboratório de Entomologia, Fiocruz Rondônia, Porto Velho, Brazil., Cella W; Programa de Pós Graduação em Ciência Animal com Ênfase em Produtos Bioativos, Universidade Paranaense, Paraná, Brazil.; Universidade do Estado do Amazonas (UEA), Tefé, Brazil., Ferreira GEM; Programa de Pós-Graduação em Biologia Experimental, Fundação Universidade Federal de Rondônia, Porto Velho, Brazil.; Laboratório de Epidemiologia Genética, Fiocruz Rondônia, Porto Velho, Brazil., de Medeiros JF; Programa de Pós-Graduação em Biologia Experimental, Fundação Universidade Federal de Rondônia, Porto Velho, Brazil.; Laboratório de Entomologia, Fiocruz Rondônia, Porto Velho, Brazil., Shimabukuro PHF; Grupo de Estudos em Leishmanioses, Instituto René Rachou, Fiocruz Minas, Belo Horizonte, Brazil.; Coleção de Flebotomíneos (Fiocruz/COLFLEB), Instituto René Rachou, Fiocruz Minas, Belo Horizonte, Brazil.
Jazyk:	angličtina
Zdroj:	Medical and veterinary entomology [Med Vet Entomol] 2024 Mar; Vol. 38 (1), pp. 83-98. Date of Electronic Publication: 2023 Oct 22.
DOI:	10.1111/mve.12701
Abstrakt:	In this study, we analysed the molecular and morphometric differences of several populations of the putative sand fly vector Psychodopygus davisi (Root, 1934) (Diptera, Psychodidae, Phlebotominae) in Brazil. We amplified the 658 base pair fragments of the DNA barcoding region-cytochrome c oxidase subunit 1 (COI) gene-for 57 specimens of P. davisi and three specimens of Psychodopygus claustrei (Abonnenc, Léger & Fauran, 1979). We merged our data with public sequences of the same species available from GenBank. Then, the combined dataset-87 sequences and 20 localities-was analysed using population structure analysis and different species delimitation approaches. Geometric morphometry of wings was performed for 155 specimens of P. davisi populations from the North, Midwest and Southeast Brazilian regions, analysing the differences in centroid sizes and canonical variates. Molecular analysis indicated high intraspecific genetic distance values for P. davisi (maximum p distance = 5.52%). All algorithms identified P. davisi and P. claustrei as distinct molecular taxonomic units, despite the low interspecific distance (p distance to the nearest neighbour = 4.79%). P. davisi sequences were split into four genetic clusters by population structure analysis and at least five genetic lineages using intermediate scenarios of the species delimitation algorithms. The species validation analysis of BPP strongly supported the five-species model in our dataset. We found high genetic diversity in this taxon, which is in agreement with its wide geographic distribution in Brazil. Furthermore, the wing analysis showed that specimens from the Southeast Region of Brazil are different from those in the North and the Midwest. The evolutionary patterns of P. davisi populations in Brazil suggest the presence of candidate species, which need to be validated in future studies using a more comprehensive approach with both genomic data and morphological characters. (© 2023 Royal Entomological Society.)
Databáze:	MEDLINE
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