Mass production of lumenogenic human embryoid bodies and functional cardiospheres using in-air-generated microcapsules.

Autor: van Loo B; University of Twente, TechMed Centre, Department of Developmental BioEngineering, Enschede, The Netherlands., Ten Den SA; University of Twente, TechMed Centre, Department of Applied Stem Cell Technology, Enschede, The Netherlands., Araújo-Gomes N; University of Twente, TechMed Centre, Department of Developmental BioEngineering, Enschede, The Netherlands., de Jong V; University of Twente, TechMed Centre, Department of Developmental BioEngineering, Enschede, The Netherlands., Snabel RR; Radboud University, Radboud Institute for Molecular Life Sciences, Faculty of Science, Department of Molecular Developmental Biology, Nijmegen, The Netherlands., Schot M; University of Twente, TechMed Centre, Department of Developmental BioEngineering, Enschede, The Netherlands., Rivera-Arbeláez JM; University of Twente, TechMed Centre, Department of Applied Stem Cell Technology, Enschede, The Netherlands.; University of Twente, TechMed Centre, Max Planck Center for Complex Fluid Dynamics, BIOS Lab-on-a-Chip Group, Enschede, The Netherlands., Veenstra GJC; Radboud University, Radboud Institute for Molecular Life Sciences, Faculty of Science, Department of Molecular Developmental Biology, Nijmegen, The Netherlands., Passier R; University of Twente, TechMed Centre, Department of Applied Stem Cell Technology, Enschede, The Netherlands.; Leiden University Medical Centre, Department of Anatomy and Embryology, Leiden, Netherlands., Kamperman T; University of Twente, TechMed Centre, Department of Developmental BioEngineering, Enschede, The Netherlands.; IamFluidics B.V., De Veldmaat 17, 7522NM, Enschede, The Netherlands., Leijten J; University of Twente, TechMed Centre, Department of Developmental BioEngineering, Enschede, The Netherlands. Jeroen.leijten@utwente.nl.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Oct 21; Vol. 14 (1), pp. 6685. Date of Electronic Publication: 2023 Oct 21.
DOI: 10.1038/s41467-023-42297-0
Abstrakt: Organoids are engineered 3D miniature tissues that are defined by their organ-like structures, which drive a fundamental understanding of human development. However, current organoid generation methods are associated with low production throughputs and poor control over size and function including due to organoid merging, which limits their clinical and industrial translation. Here, we present a microfluidic platform for the mass production of lumenogenic embryoid bodies and functional cardiospheres. Specifically, we apply triple-jet in-air microfluidics for the ultra-high-throughput generation of hollow, thin-shelled, hydrogel microcapsules that can act as spheroid-forming bioreactors in a cytocompatible, oil-free, surfactant-free, and size-controlled manner. Uniquely, we show that microcapsules generated by in-air microfluidics provide a lumenogenic microenvironment with near 100% efficient cavitation of spheroids. We demonstrate that upon chemical stimulation, human pluripotent stem cell-derived spheroids undergo cardiomyogenic differentiation, effectively resulting in the mass production of homogeneous and functional cardiospheres that are responsive to external electrical stimulation. These findings drive clinical and industrial adaption of stem cell technology in tissue engineering and drug testing.
(© 2023. Springer Nature Limited.)
Databáze: MEDLINE
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