Chlorophyll biosynthesis under the control of arginine metabolism.

Autor:	Kiss É; Laboratory of Photosynthesis, Centre Algatech, Institute of Microbiology, The Czech Academy of Sciences, 37901 Třeboň, Czech Republic., Talbot J; Laboratory of Photosynthesis, Centre Algatech, Institute of Microbiology, The Czech Academy of Sciences, 37901 Třeboň, Czech Republic., Adams NBP; NanoTemper Technologies, Floessegasse 4, 81369 Munich, Germany; Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK., Opekar S; Biology Centre of the Czech Academy of Sciences, Branišovská 1160/31, 370 05 České Budějovice, Czech Republic., Moos M; Biology Centre of the Czech Academy of Sciences, Branišovská 1160/31, 370 05 České Budějovice, Czech Republic., Pilný J; Laboratory of Photosynthesis, Centre Algatech, Institute of Microbiology, The Czech Academy of Sciences, 37901 Třeboň, Czech Republic., Kvasov T; NanoTemper Technologies, Floessegasse 4, 81369 Munich, Germany., Schneider E; NanoTemper Technologies, Floessegasse 4, 81369 Munich, Germany., Koník P; Laboratory of Photosynthesis, Centre Algatech, Institute of Microbiology, The Czech Academy of Sciences, 37901 Třeboň, Czech Republic; Faculty of Science, University of South Bohemia, 37005 České Budějovice, Czech Republic., Šimek P; Biology Centre of the Czech Academy of Sciences, Branišovská 1160/31, 370 05 České Budějovice, Czech Republic., Sobotka R; Laboratory of Photosynthesis, Centre Algatech, Institute of Microbiology, The Czech Academy of Sciences, 37901 Třeboň, Czech Republic; Faculty of Science, University of South Bohemia, 37005 České Budějovice, Czech Republic. Electronic address: sobotka@alga.cz.
Jazyk:	angličtina
Zdroj:	Cell reports [Cell Rep] 2023 Nov 28; Vol. 42 (11), pp. 113265. Date of Electronic Publication: 2023 Oct 20.
DOI:	10.1016/j.celrep.2023.113265
Abstrakt:	In natural environments, photosynthetic organisms adjust their metabolism to cope with the fluctuating availability of combined nitrogen sources, a growth-limiting factor. For acclimation, the dynamic degradation/synthesis of tetrapyrrolic pigments, as well as of the amino acid arginine, is pivotal; however, there has been no evidence that these processes could be functionally coupled. Using co-immunopurification and spectral shift assays, we found that in the cyanobacterium Synechocystis sp. PCC 6803, the arginine metabolism-related ArgD and CphB enzymes form protein complexes with Gun4, an essential protein for chlorophyll biosynthesis. Gun4 binds ArgD with high affinity, and the Gun4-ArgD complex accumulates in cells supplemented with ornithine, a key intermediate of the arginine pathway. Elevated ornithine levels restricted de novo synthesis of tetrapyrroles, which arrested the recovery from nitrogen deficiency. Our data reveal a direct crosstalk between tetrapyrrole biosynthesis and arginine metabolism that highlights the importance of balancing photosynthetic pigment synthesis with nitrogen homeostasis. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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