A Drosophila glial cell atlas reveals a mismatch between transcriptional and morphological diversity.

Autor: Lago-Baldaia I; Department of Cell and Developmental Biology, University College London, London, United Kingdom., Cooper M; Department of Cell and Developmental Biology, University College London, London, United Kingdom., Seroka A; Institute of Neuroscience, Howard Hughes Medical Institute, University of Oregon, Eugene, Oregon, United States of America., Trivedi C; Department of Cell and Developmental Biology, University College London, London, United Kingdom., Powell GT; Department of Cell and Developmental Biology, University College London, London, United Kingdom., Wilson SW; Department of Cell and Developmental Biology, University College London, London, United Kingdom., Ackerman SD; Department of Pathology and Immunology, Brain Immunology and Glia Center, Washington University School of Medicine, Saint Louis, Missouri, United States of America.; Department of Developmental Biology, Washington University School of Medicine, Saint Louis, Missouri, United States of America., Fernandes VM; Department of Cell and Developmental Biology, University College London, London, United Kingdom.
Jazyk: angličtina
Zdroj: PLoS biology [PLoS Biol] 2023 Oct 20; Vol. 21 (10), pp. e3002328. Date of Electronic Publication: 2023 Oct 20 (Print Publication: 2023).
DOI: 10.1371/journal.pbio.3002328
Abstrakt: Morphology is a defining feature of neuronal identity. Like neurons, glia display diverse morphologies, both across and within glial classes, but are also known to be morphologically plastic. Here, we explored the relationship between glial morphology and transcriptional signature using the Drosophila central nervous system (CNS), where glia are categorised into 5 main classes (outer and inner surface glia, cortex glia, ensheathing glia, and astrocytes), which show within-class morphological diversity. We analysed and validated single-cell RNA sequencing data of Drosophila glia in 2 well-characterised tissues from distinct developmental stages, containing distinct circuit types: the embryonic ventral nerve cord (VNC) (motor) and the adult optic lobes (sensory). Our analysis identified a new morphologically and transcriptionally distinct surface glial population in the VNC. However, many glial morphological categories could not be distinguished transcriptionally, and indeed, embryonic and adult astrocytes were transcriptionally analogous despite differences in developmental stage and circuit type. While we did detect extensive within-class transcriptomic diversity for optic lobe glia, this could be explained entirely by glial residence in the most superficial neuropil (lamina) and an associated enrichment for immune-related gene expression. In summary, we generated a single-cell transcriptomic atlas of glia in Drosophila, and our extensive in vivo validation revealed that glia exhibit more diversity at the morphological level than was detectable at the transcriptional level. This atlas will serve as a resource for the community to probe glial diversity and function.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Lago-Baldaia et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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