Molecular analysis of canine circovirus in dogs from animal shelters in Belém, Pará, northern Brazil: first detection at the amazon region.

Autor:	Cardoso BTM; Biomedical Scientist, Programa de Pós-graduação em Biologia Parasitária da Amazônia (PPGBPA), Centro de Ciências Biológicas e da Saúde (CCBS), Universidade do Estado do Pará, Belém, PA, Brazil., de Deus DR; Biologist, MSc., Programa de Pós-graduação em Virologia, Instituto Evandro Chagas, Ananindeua, PA, Brazil., Sousa EC; Biomedical Scientist, DSc., Seção de Parasitologia, Instituto Evandro Chagas, Ananindeua, PA, Brazil., Pinheiro KDC; Biomedical Scientist, DSc., Seção de Virologia, Instituto Evandro Chagas, Ananindeua, PA, Brazil., da Costa JN; Biomedical Scientist, Programa de Pós-graduação em Biologia Parasitária da Amazônia (PPGBPA), Centro de Ciências Biológicas e da Saúde (CCBS), Universidade do Estado do Pará, Belém, PA, Brazil., Maués MAC; Veterinarian, DSc., Centro de Controle de Zoonoses do Pará, Belém, PA, Brazil., Mesquita MJF; Veterinarian, DSc., Universidade Rural da Amazônia, Belém, PA, Brazil., Teixeira DM; Biomedical Scientist, DSc., Seção de Virologia, Instituto Evandro Chagas, Ananindeua, PA, Brazil., Siqueira JAM; Biomedical Scientist, DSc., Seção de Virologia, Instituto Evandro Chagas, Ananindeua, PA, Brazil., Resque HR; Biomedical Scientist, DSc., Seção de Virologia, Instituto Evandro Chagas, Ananindeua, PA, Brazil., Gabbay YB; Biomedical Scientist, DSc., Seção de Virologia, Instituto Evandro Chagas, Ananindeua, PA, Brazil., da Silva LD; Biomedical Scientist, DSc., Seção de Virologia, Instituto Evandro Chagas, Ananindeua, PA, Brazil.
Jazyk:	angličtina
Zdroj:	Brazilian journal of veterinary medicine [Braz J Vet Med] 2023 Oct 03; Vol. 45, pp. e000723. Date of Electronic Publication: 2023 Oct 03 (Print Publication: 2023).
DOI:	10.29374/2527-2179.bjvm000723
Abstrakt:	The canine circovirus (CanCV) is a single-stranded DNA virus that has become an important emerging virus associated with gastroenteritis in dogs worldwide. In the present study, the CanCV was detected by PCR in 15% (22/147) of dogs from animal shelters in Belém, between 2019 and 2020. We observed an association between the CanCV infection and the presence of diarrhea in animals younger than one year of age (p > 0.01). The Brazilian strains were grouped in Chinese genotypes, with 99.54 to 100% nucleotilde homology. The GMRF Bayesian Skyride used the molecular clock model, which was the best suited technique to plot the dataset. The most recent common ancestor (TMRCA) was estimated in 2017, with the evolution rate of 1.6 x 10 -3 s/s/y. The viral family diversity was also investigated, with emphasis on the families of the enteric pathogenic viruses Parvoviridae, Picornaviridae and Astroviridae, which were detected in the CanCV positive pooled samples. This study highlights the importance of the CanCV as an emergent virus that causes diarrhea in Brazilian dogs. The results found herein contribute to the understanding of the role of CanCV in enteric diseases and in the evolutionary molecular characterization of the circulating genotypes. Furthermore, we increased the understanding of the fecal virome in dogs with diarrhea, providing data for the monitoring and prevention viral gastroenteric diseases in domestic animals. Competing Interests: Conflict of interests: BTMC, ECSJ, KCP, JCN, DMT, HRR, DRD, JAMS, YBG and LDS have no conflict of interest.
Databáze:	MEDLINE
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