Collagen crosslinking impacts stromal wound healing and haze formation in a rabbit phototherapeutic keratectomy model.

Autor:	Moore BA; Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL., Jalilian I; Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California-Davis, Davis, CA., Kim S; Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California-Davis, Davis, CA., Mizutani M; Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California-Davis, Davis, CA., Mukai M; Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California-Davis, Davis, CA., Chang C; Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California-Davis, Davis, CA., Entringer AM; Department of Biomedical Engineering, Cullen College of Engineering, University of Houston, Houston, TX., Dhamodaran K; Department of Basic Sciences, College of Optometry, University of Houston, Houston, TX., Raghunathan VK; Department of Biomedical Engineering, Cullen College of Engineering, University of Houston, Houston, TX.; Department of Basic Sciences, College of Optometry, University of Houston, Houston, TX., Teixeira LBC; Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI., Murphy CJ; Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California-Davis, Davis, CA.; Department of Ophthalmology & Vision Science, School of Medicine, University of California-Davis, Sacramento, CA., Thomasy SM; Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California-Davis, Davis, CA.; Department of Ophthalmology & Vision Science, School of Medicine, University of California-Davis, Sacramento, CA.
Jazyk:	angličtina
Zdroj:	Molecular vision [Mol Vis] 2023 Jul 16; Vol. 29, pp. 102-116. Date of Electronic Publication: 2023 Jul 16 (Print Publication: 2023).
Abstrakt:	Purpose: The purpose of this study was to evaluate the elastic modulus, keratocyte-fibroblast-myocyte transformation, and haze formation of the corneal stroma following combined phototherapeutic keratectomy (PTK) and epithelium-off UV-A/riboflavin corneal collagen crosslinking (CXL) using an in vivo rabbit model. Methods: Rabbits underwent PTK and CXL, PTK only, or CXL 35 days before PTK. Rebound tonometry, Fourier-domain optical coherence tomography, and ultrasound pachymetry were performed on days 7, 14, 21, 42, 70, and 90 post-operatively. Atomic force microscopy, histologic inflammation, and immunohistochemistry for α-smooth muscle actin (α-SMA) were assessed post-mortem. Results: Stromal haze formation following simultaneous PTK and CXL was significantly greater than in corneas that received PTK only and persisted for more than 90 days. No significant difference in stromal haze was noted between groups receiving simultaneous CXL and PTK and those receiving CXL before PTK. Stromal inflammation did not differ between groups at any time point, although the intensity of α-SMA over the number of nuclei was significantly greater at day 21 between groups receiving simultaneous CXL and PTK and those receiving CXL before PTK. The elastic modulus was significantly greater in corneas receiving simultaneous CXL and PTK compared with those receiving PTK alone. Conclusions: We showed that stromal haze formation and stromal stiffness is significantly increased following CXL, regardless of whether it is performed at or before the time of PTK. Further knowledge of the biophysical cues involved in determining corneal wound healing duration and outcomes will be important for understanding scarring following CXL and for the development of improved therapeutic options. (Copyright © 2023 Molecular Vision.)
Databáze:	MEDLINE
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