Protective efficacy of Toxoplasma gondii infected cells-derived exosomes against chronic murine toxoplasmosis.

Autor: Tawfeek GM; Medical Parasitology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt., Abou-El-Naga IF; Medical Parasitology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt. Electronic address: eman.abuelnag@alexmed.edu.eg., Hassan EME; Medical Parasitology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt., Sabry D; Medical Biochemistry and Molecular Biology, Faculty of Medicine, Badr University in Cairo, Egypt; Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Egypt., Meselhey RA; Medical Parasitology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt., Younis SS; Medical Parasitology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Jazyk: angličtina
Zdroj: Acta tropica [Acta Trop] 2023 Dec; Vol. 248, pp. 107041. Date of Electronic Publication: 2023 Oct 17.
DOI: 10.1016/j.actatropica.2023.107041
Abstrakt: Exosomes were isolated from T. gondii infected human hepatoblastoma cells using the exosome isolation kit and characterized by electron microscopy and Western blotting. Exosomes adsorbed to alum adjuvant were evaluated as a potential immunizing agent against murine chronic toxoplasmosis compared to excretory secretory antigens (ESA)-alum. Mice were immunized at days 1, 15 and 29. The levels of IgG, IFN-γ, IL-4 and IL-10, CD4 + and CD8 + T cells were determined using sandwich enzyme-linked immunosorbent assay (sandwich ELISA) at days 14, 28 and 56 of the experiment. Then mice were infected orally with 10 cysts of T. gondii. The protective efficacy of the antigens were evaluated by counting the brain cysts and measuring the aforementioned humoral and cellular parameters 60 days post infection. The results showed that alum increased the protective efficacy of the exosomes. Immunization with exosome-alum induced both humoral and mixed Th1/Th2 cellular immune responses. Exosome-alum gave higher levels of the humoral and cellular parameters, compared to ESA-alum. After challenge infection, exosome-alum significantly reduced the brain cyst burden by 75 % while ESA-alum gave 42 % reduction and evoked higher humoral and cellular immune responses. Therefore, the possibility of using T. gondii infected cells-derived exosome-alum as a vaccine is a new perspective in toxoplasmosis.
Competing Interests: Declaration of Competing Interest The authors declare that there are no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: