Therapeutic potential of lactoferrin-coated iron oxide nanospheres for targeted hyperthermia in gastric cancer.

Autor: Attri K; School of Chemistry and Biochemistry, Thapar Institute of Engineering and Technology, Patiala, Punjab, 147004, India.; TIET-VT Centre of Excellence for Emerging Materials, Thapar Institute of Engineering and Technology, Patiala, Punjab, 147004, India., Chudasama B; School of Physics and Material Sciences, Thapar Institute of Engineering and Technology, Patiala, Punjab, 147004, India. bnchudasama@thapar.edu.; TIET-VT Centre of Excellence for Emerging Materials, Thapar Institute of Engineering and Technology, Patiala, Punjab, 147004, India. bnchudasama@thapar.edu., Mahajan RL; Department of Mechanical Engineering, Department of Materials Science and Engineering, Virginia Tech, Blacksburg, VA, 24061, USA. mahajanr@vt.edu.; TIET-VT Centre of Excellence for Emerging Materials, Thapar Institute of Engineering and Technology, Patiala, Punjab, 147004, India. mahajanr@vt.edu., Choudhury D; School of Chemistry and Biochemistry, Thapar Institute of Engineering and Technology, Patiala, Punjab, 147004, India. diptiman@thapar.edu.; TIET-VT Centre of Excellence for Emerging Materials, Thapar Institute of Engineering and Technology, Patiala, Punjab, 147004, India. diptiman@thapar.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Oct 19; Vol. 13 (1), pp. 17875. Date of Electronic Publication: 2023 Oct 19.
DOI: 10.1038/s41598-023-43725-3
Abstrakt: Lactoferrin (LF) is a non-heme iron-binding glycoprotein involved in the transport of iron in blood plasma. In addition, it has many biological functions, including antibacterial, antiviral, antimicrobial, antiparasitic, and, importantly, antitumor properties. In this study, we have investigated the potential of employing lactoferrin-iron oxide nanoparticles (LF-IONPs) as a treatment modality for gastric cancer. The study confirms the formation of LF-IONPs with a spherical shape and an average size of 5 ± 2 nm, embedded within the protein matrix. FTIR and Raman analysis revealed that the Fe-O bond stabilized the protein particle interactions. Further, we conducted hyperthermia studies to ascertain whether the proposed composite can generate a sufficient rise in temperature at a low frequency. The results confirmed that we can achieve a temperature rise of about 7 °C at 242.4 kHz, which can be further harnessed for gastric cancer treatment. The particles were further tested for their anti-cancer activity on AGS cells, with and without hyperthermia. Results indicate that LF-IONPs (10 µg/ml) significantly enhance cytotoxicity, resulting in the demise of 67.75 ± 5.2% of cells post hyperthermia, while also exhibiting an inhibitory effect on cell migration compared to control cells, with the most inhibition observed after 36 h of treatment. These findings suggest the potential of LF-IONPs in targeted hyperthermia treatment of gastric cancer.
(© 2023. Springer Nature Limited.)
Databáze: MEDLINE
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