Low-dose lipopolysaccharide exposure during oocyte maturation disrupts early bovine embryonic development.

Autor: Castro B; Department of Animal and Range Sciences, New Mexico State University, Las Cruces, NM, 88003, USA., Candelaria JI; Department of Animal Science, University of California, Davis, CA, 95616, USA., Austin MM; Department of Animal and Range Sciences, New Mexico State University, Las Cruces, NM, 88003, USA., Shuster CB; Department of Biology, New Mexico State University, Las Cruces, NM, 88003, USA., Gifford CA; Extension Animal Sciences and Natural Resources, New Mexico State University, Las Cruces, NM, 88003, USA., Denicol AC; Department of Animal Science, University of California, Davis, CA, 95616, USA., Hernandez Gifford JA; Department of Animal and Range Sciences, New Mexico State University, Las Cruces, NM, 88003, USA. Electronic address: jgifford@nmsu.edu.
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2024 Jan 15; Vol. 214, pp. 57-65. Date of Electronic Publication: 2023 Oct 12.
DOI: 10.1016/j.theriogenology.2023.10.010
Abstrakt: Gram-negative bacteria release of lipopolysaccharide (LPS) endotoxin elicits robust immune responses capable of disrupting normal ovarian function contributing to female infertility. However, effects of subclinical or non-detectable infections on oocyte competence and subsequent embryo development remain to be fully elucidated. The aim of this study was to investigate the effects of exposing bovine oocytes to low LPS doses on oocyte and embryo competence. Bovine oocytes were collected from slaughterhouse-derived ovaries and matured with vehicle-control or increasing doses of LPS (0.01, 0.1, and 1 μg/mL) for 21 h. Oocytes (n = 252) were evaluated for nuclear maturation. A set of embryos from LPS-matured oocytes (n = 300) were cultured for 8 d to evaluate day 3 cleavage rates and day 8 blastocyst rates along with blastocyst cell counts. A subset of oocytes (n = 153) was fertilized and cultured for time-lapse image capture and analysis of embryo development. Results demonstrate no significant treatment differences among treatment groups in percent of oocytes at germinal vesicle (GV; P = 0.90), germinal vesicle breakdown (GVBD; P = 0.13), meiosis I (MI; P = 0.26), or metaphase II (MII; P = 0.44). Likewise, treatment differences were not observed in cleavage rates (P = 0.97), or blastocyst rates (P = 0.88) evaluated via traditional microscopy. Treatment with LPS did not affect total blastocyst cell count (P = 0.68), as indicated by trophectoderm (P = 0.83), and inner cell mass (P = 0.21) cell counts. Time-lapse embryo evaluation demonstrated no differences among control or LPS matured oocytes in number of zygotes that did not cleave after fertilization (P = 0.84), or those that cleaved but arrested at the 2-cell stage (P = 0.50), 4-cell (P = 0.76), prior to morula (P = 0.76). However, embryos derived from oocytes challenged with 0.1 μg/mL LPS tended to have reduced development to the morula stage compared with vehicle-treated controls (P = 0.06). Additionally, the percentage of blastocysts derived from oocytes matured in 0.01 μg/mL LPS tended to decrease compared to vehicle-treated controls (11.38 and 25.45 %, respectively; P = 0.09). Similarly, the proportion of oocytes that developed to the blastocyst stage was greater in vehicle-treated controls (25.45 %) compared with embryos derived from oocytes matured in 0.1 and 1 μg/mL (5.92 and 6.55 %, respectively; P = 0.03) LPS. These data suggest LPS-matured oocytes that subsequently underwent in vitro fertilization, experienced decreased competence to develop to the blastocyst stage.
Competing Interests: Declaration of competing interest None.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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