A Fast, Robust Method for Quantitative Assessment of Collagen Fibril Architecture from Transmission Electron Micrographs.
| Autor: | Rego BV; Department of Biomedical Engineering, Yale University, 55 Prospect Street, New Haven, CT 06511, USA.; Department of Biological & Agricultural Engineering, Louisiana State University, 149 E. B. Doran Building, Baton Rouge, LA 70803, USA., Weiss D; Department of Biomedical Engineering, Yale University, 55 Prospect Street, New Haven, CT 06511, USA., Humphrey JD; Department of Biomedical Engineering, Yale University, 55 Prospect Street, New Haven, CT 06511, USA.; Vascular Biology and Therapeutics Program, Yale School of Medicine, 10 Amistad Street, New Haven, CT 06520, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada [Microsc Microanal] 2023 Dec 21; Vol. 29 (6), pp. 2099-2107. |
| DOI: | 10.1093/micmic/ozad116 |
| Abstrakt: | Collagen is the most abundant protein in mammals; it exhibits a hierarchical organization and provides structural support to a wide range of soft tissues, including blood vessels. The architecture of collagen fibrils dictates vascular stiffness and strength, and changes therein can contribute to disease progression. While transmission electron microscopy (TEM) is routinely used to examine collagen fibrils under normal and pathological conditions, computational tools that enable fast and minimally subjective quantitative assessment remain lacking. In the present study, we describe a novel semi-automated image processing and statistical modeling pipeline for segmenting individual collagen fibrils from TEM images and quantifying key metrics of interest, including fibril cross-sectional area and aspect ratio. For validation, we show first-of-their-kind illustrative results for adventitial collagen in the thoracic aorta from three different mouse models. (© The Author(s) 2023. Published by Oxford University Press on behalf of the Microscopy Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.) |
| Databáze: | MEDLINE |
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