L-glutamine supplementation reduced morphological damage in the renal glomerulus of rats with Walker-256 tumor.

Autor:	Lima KRA; Universidade Federal do Rio Grande do Norte - Natal (Rio Grande do Norte) - Brazil., Lopes MLDS; Universidade Federal do Rio Grande do Norte - Natal (Rio Grande do Norte) - Brazil., Souza SRG; Universidade Estadual de Maringá - Maringá (Paraná) - Brazil., Fracaro L; Universidade Estadual de Maringá - Maringá (Paraná) - Brazil., Purificação NRCD; Universidade Federal do Rio Grande do Norte - Natal (Rio Grande do Norte) - Brazil., Lima MFO; Universidade Federal do Rio Grande do Norte - Natal (Rio Grande do Norte) - Brazil., Lins LAA; Universidade Federal do Rio Grande do Norte - Natal (Rio Grande do Norte) - Brazil., Lacchini S; Universidade de São Paulo - São Paulo (São Paulo) - Brazil., Araújo AA; Universidade Federal do Rio Grande do Norte - Natal (Rio Grande do Norte) - Brazil., Araújo Júnior RF; Universidade Federal do Rio Grande do Norte - Natal (Rio Grande do Norte) - Brazil., Perles JVCM; Universidade Estadual de Maringá - Maringá (Paraná) - Brazil., Zanoni JN; Universidade Estadual de Maringá - Maringá (Paraná) - Brazil., Clebis NK; Universidade Federal do Rio Grande do Norte - Natal (Rio Grande do Norte) - Brazil.
Jazyk:	angličtina
Zdroj:	Acta cirurgica brasileira [Acta Cir Bras] 2023 Oct 13; Vol. 38, pp. e383923. Date of Electronic Publication: 2023 Oct 13 (Print Publication: 2023).
DOI:	10.1590/acb383923
Abstrakt:	Purpose: To evaluate the effects of the experimental subcutaneous Walker-256 tumor and L-glutamine supplementation, an antioxidant, on the glomerular morphology of rats. Methods: Twenty Wistar rats were distributed into four groups (n = 5): control (C); control treated with 2% L-glutamine (CG); rats with Walker-256 tumor (WT); and rats with Walker-256 tumor treated with 2% L-glutamine (WTG). Renal histological samples were submitted to periodic acid-Schiff and Masson's Trichrome staining to analyze glomerular density, morphometry of glomerular components and glomerulosclerosis; and to immunohistochemistry for fibroblast growth factor-2 (FGF-2). Results: WT showed 50% reduction in body mass gain and cachexia index > 10%, while WTG demonstrated reduction in cachexia (p < 0.05). WT revealed reduction of glomerular density, increase in the glomerular tuft area, mesangial area, matrix in the glomerular tuft, decrease in the urinary space and synechia, and consequently higher glomerulosclerosis (p < 0.05). L-glutamine supplementation in the WTG improved glomerular density, and reduced glomerular tuft area, urinary space, mesangial area, and glomerulosclerosis compared to WT(p < 0.05). WT showed higher collagen area and FGF-2 expression compared to C (p < 0.05). WTG presented lower collagen fibers and FGF-2 expression compared to WT (p < 0.05). Conclusions: L-glutamine supplementation reduced cachexia and was beneficial for glomerular morphology of the rats, as well as it reduced kidney damage and improved the remaining glomeruli morphology.
Databáze:	MEDLINE
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