| Autor: |
Singh R; ICAR-Indian Veterinary Research Institute, Bareilly, India., Singh KP; ICAR-Indian Veterinary Research Institute, Bareilly, India., Singh R; SOA Institute of Veterinary Science and Animal Husbandry, Bhubaneswar, India., Singh V; ICAR-Indian Veterinary Research Institute, Bareilly, India., Kumar P; ICAR-Indian Veterinary Research Institute, Bareilly, India., Varshney R; Department of Veterinary Microbiology, Faculty of Veterinary and Animal Sciences, I.Ag.Scs, RGSC, Banaras Hindu University, Mirzapur, India., Yadav A; ICAR-Indian Veterinary Research Institute, Bareilly, India., Mote A; ICAR-Indian Veterinary Research Institute, Bareilly, India., Gangwar M; ICAR-Indian Veterinary Research Institute, Bareilly, India., Prasath NB; ICAR-Indian Veterinary Research Institute, Bareilly, India. |
| Abstrakt: |
Bluetongue virus (BTV), a major peril to the sheep industry, infects a wide range of the cells in the infected animals including mononuclear, dendritic and epithelial cells. However, little is known about its tropism for the secretory epithelial cells of endocrine glands and the pathogenesis it induces. The aim of the study was to assess the BTV load, antigen distribution in the tissue of the pituitary, thyroid as well as adrenal glands and associated histopathological consequences. BTV antigens were localized using immunohistochemistry in the thyroid's epithelial cells, zona fasciculata and zona reticularis cells and the anterior pituitary epithelial cells. The real-time PCR portrayed the high viral load in adrenals at 7 th days postinoculation (DPI) and in thyroid and pituitary glands at 15 th DPI. Serum examination revealed variation in the T-3 and T-4 of infected animals in comparison to the control group. Caspase-3 immunolocalization revealed BTV-1 induces apoptosis in the affected cells of endocrine gland of infected animals. Further, this study signifies the tropism of BTV in the novel sites (endocrine glands) of the host that might be one of the reasons for the poor performance of infected animals. |
