[Effect of Fitofron on the persistence factors of opportunistic pathogens in urine isolated from patients with urinary tract infections].

Autor: Pashkova TM; FGBUN Orenburg Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Orenburg, Russia., Pashinina OA; FGBUN Orenburg Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Orenburg, Russia., Kuzmin MD; FGBUN Orenburg Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Orenburg, Russia., Kartashova OL; FGBUN Orenburg Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Orenburg, Russia.
Jazyk: ruština
Zdroj: Urologiia (Moscow, Russia : 1999) [Urologiia] 2023 Sep (4), pp. 53-57.
Abstrakt: Aim: To perform an experimental evaluation of the effect of Phytofron, used for the treatment of urinary tract infections, on the ability of opportunistic pathogens to inactivate innate immunity factors (lysozyme, pro- and anti-inflammatory cytokines) and form biofilms.
Materials and Methods: In vitro experiments were carried out on clinical isolates from urine of patients with pyelonephritis and cystitis: Escherichia coli, Staphylococcus aureus, S. haemolyticus, S. epidermidis, Enterococcus faecalis. The effect of Fitofron NPO FarmVILAR (Russia) on the anticytokine activity of bacteria against regulatory cytokines (IL4, IL6, IL8, TNF and IL17A) was determined by enzyme immunoassay, while anti-lysozyme trait and the ability to form biofilms was evaluated by the photometric method.
Results: The inhibitory effect of Fitofron on the ability of opportunistic microorganisms to inactivate innate immunity factors (lysozyme) and form biofilms, as well as the predominant inhibition of the studied cytokines, was experimentally established.
Conclusion: Inhibition of the persistence factors of opportunistic pathogens by Fitofron, documented in vitro, can be considered as one of the possible mechanisms of its biological activity in vivo.
Databáze: MEDLINE
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