Engineering a Circular Riboregulator in Escherichia coli .

Autor:	Rostain W; Warwick Integrative Synthetic Biology Centre (WISB) and School of Life Sciences, University of Warwick, CV4 7AL Coventry, UK.; Institute of Systems and Synthetic Biology, CNRS-Université d'Évry Val-d'Essonne, 91000 Évry, France., Shen S; Institute of Systems and Synthetic Biology, CNRS-Université d'Évry Val-d'Essonne, 91000 Évry, France., Cordero T; Warwick Integrative Synthetic Biology Centre (WISB) and School of Life Sciences, University of Warwick, CV4 7AL Coventry, UK.; Instituto de Biología Molecular y Celular de Plantas, CSIC-Universidad Politécnica de Valencia, 46022 Valencia, Spain., Rodrigo G; Institute of Systems and Synthetic Biology, CNRS-Université d'Évry Val-d'Essonne, 91000 Évry, France.; Instituto de Biología Molecular y Celular de Plantas, CSIC-Universidad Politécnica de Valencia, 46022 Valencia, Spain.; Institute for Integrative Systems Biology (I2SysBio), CSIC-Universitat de València, 46980 Paterna, Spain., Jaramillo A; Warwick Integrative Synthetic Biology Centre (WISB) and School of Life Sciences, University of Warwick, CV4 7AL Coventry, UK.; Institute of Systems and Synthetic Biology, CNRS-Université d'Évry Val-d'Essonne, 91000 Évry, France.; Institute for Integrative Systems Biology (I2SysBio), CSIC-Universitat de València, 46980 Paterna, Spain.
Jazyk:	angličtina
Zdroj:	Biodesign research [Biodes Res] 2020 Sep 12; Vol. 2020, pp. 1916789. Date of Electronic Publication: 2020 Sep 12 (Print Publication: 2020).
DOI:	10.34133/2020/1916789
Abstrakt:	RNAs of different shapes and sizes, natural or synthetic, can regulate gene expression in prokaryotes and eukaryotes. Circular RNAs have recently appeared to be more widespread than previously thought, but their role in prokaryotes remains elusive. Here, by inserting a riboregulatory sequence within a group I permuted intron-exon ribozyme, we created a small noncoding RNA that self-splices to produce a circular riboregulator in Escherichia coli . We showed that the resulting riboregulator can trans -activate gene expression by interacting with a cis -repressed messenger RNA. We characterized the system with a fluorescent reporter and with an antibiotic resistance marker, and we modeled this novel posttranscriptional mechanism. This first reported example of a circular RNA regulating gene expression in E. coli adds to an increasing repertoire of RNA synthetic biology parts, and it highlights that topological molecules can play a role in the case of prokaryotic regulation. Competing Interests: The authors declare that there is no conflict of interest regarding the publication of this article. (Copyright © 2020 William Rostain et al.)
Databáze:	MEDLINE
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