Performance evaluation of in-house developed Covid-19 IgG/IgM antibody rapid diagnostic kit.

Autor: G VCVS; Genomix CARL Pvt. Ltd, Pulivendula, Andhra Pradesh, 516 390, India.; Department of Biotechnology, Vignan's Foundation for Science, Technology & Research Deemed to be University, Vadlamudi, Guntur, Andhra Pradesh, 522 213, India., Reddy PVJ; Genomix CARL Pvt. Ltd, Pulivendula, Andhra Pradesh, 516 390, India., Suravajhala P; Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Clappana, Kerala, 690525, India. prash@bioclues.org.; Bioclues.org, Hyderabad, India. prash@bioclues.org., Suravajhala R; Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Clappana, Kerala, 690525, India., V UK; Genomix CARL Pvt. Ltd, Pulivendula, Andhra Pradesh, 516 390, India., Pb KK; Department of Genetics, Osmania University, Hyderabad, Telangana, 500 007, India., Tc V; Department of Biotechnology, Vignan's Foundation for Science, Technology & Research Deemed to be University, Vadlamudi, Guntur, Andhra Pradesh, 522 213, India., Polavarapu R; Genomix CARL Pvt. Ltd, Pulivendula, Andhra Pradesh, 516 390, India. giri@genomixbiotech.com.
Jazyk: angličtina
Zdroj: AMB Express [AMB Express] 2023 Oct 17; Vol. 13 (1), pp. 116. Date of Electronic Publication: 2023 Oct 17.
DOI: 10.1186/s13568-023-01620-0
Abstrakt: In the interest of preventing the Coronavirus Disease 2019 (COVID-19) pandemic from spreading, it is crucial to promptly identify and confine afflicted patients. Serological antibody testing is a significant diagnostic technique that is increasingly employed in clinics, however its clinical use is still being investigated. The present study was carried out to scrutinize how well Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) antibody testing using in-house developed rapid antibody assay worked against the chemiluminescence (CLIA) assay. Either IgG positive (IgG + IgM-) or IgM positive (IgM + IgG-); both IgG and IgM positive (IgM + IgG+); and negatives (IgM- IgG-) have been evaluated. A total of 300 samples with diverse age and sexual identity data were included. The combined sensitivities for IgG + IgM+, IgM + IgG-, IgG + IgM- and IgG-IgM- were evaluated. More accurate diagnostic results may be obtained using molecular diagnostic tools. The Antibody Rapid Diagnostic kit's (in-house developed) performance was satisfactory for determining the presence of Covid-19 infection with IgG and IgM positivity. The IgG and IgM positivity helped evaluate the immune response in the individual for the COVID-19 infection. These results lend support to the additional utilisation of serological antibody tests in the COVID-19 diagnosis.
(© 2023. Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje