Green UPLC method for estimation of ciprofloxacin, diclofenac sodium, and ibuprofen with application to pharmacokinetic study of human samples.

Autor: Ahmed-Anwar AA; Chemistry Department, Faculty of Science, Beni-Suef University, Beni-Suef, 62514, Egypt.; Central Research Laboratory, Analytical Chemistry Department, Nahda University, Beni-Suef, Egypt., Mohamed MA; Hikma Pharmaceutical Company, Beni-Suef, Egypt., Farghali AA; Materials Science and Nanotechnology Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Beni-Suef, Egypt., Mahmoud R; Chemistry Department, Faculty of Science, Beni-Suef University, Beni-Suef, 62514, Egypt., Hassouna MEM; Chemistry Department, Faculty of Science, Beni-Suef University, Beni-Suef, 62514, Egypt. Mohamed.hassouna@science.bsu.edu.eg.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Oct 17; Vol. 13 (1), pp. 17613. Date of Electronic Publication: 2023 Oct 17.
DOI: 10.1038/s41598-023-44846-5
Abstrakt: Investigation of a unique and fast method for the determination and separation of a mixture of three drugs viz., ciprofloxacin (CIP), Ibuprofen (IBU), and diclofenac sodium (DIC) in actual samples of human plasma. Also, the technique was used to look at their pharmacokinetics study. Hydrocortisone was chosen as the internal standard (IS). The drugs were chromatographically separated using an Acquity ultra-performance liquid chromatography UPLC ® BEH C18 1.7 µm (2.1 × 150 mm) column with a mobile phase composed of acetonitrile: water (65:35, v/v) adjusted to pH 3 with diluted acetic acid. Plasma proteins were precipitated with acetonitrile. The separated drugs ranged from 0.3 to 10, 0.2-11, and 1-25 µg/mL for CIP, IBU, and DIC, respectively. Calibration curves were discovered to achieve linearity with acceptable correlation coefficients (0.99%). Examination of quality assurance samples showed exceptional precision and accuracy. Following the successful application of this improved technique to plasma samples, the pharmacokinetic characteristics of each selected drug were evaluated using (UPLC) with UV detection at 210 nm. Two green metrics were applied, the Analytical Eco-scale and the Analytical GREEnness Calculator (AGREE). Separation was achieved in only 4-min analysis time. The method's validation agreed with the requirements of the FDA, and the results were sufficient.
(© 2023. Springer Nature Limited.)
Databáze: MEDLINE
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