Efficacy of a Mexican folk remedy containing cuachalalate (Amphipterygium adstringens (Schltdl.) Schiede ex Standl) for the treatment of burn wounds infected with Pseudomonas aeruginosa.

Autor: Gómez-Salgado MDRH; Laboratorio de Investigación y Aplicación de Fitoquímicos Bioactivos, Posgrado en Botánica, Colegio de Postgraduados, Texcoco, 56230, Mexico. Electronic address: hortencia.gomez@issste.gob.mx., Beltrán-Gómez JÁ; Laboratorio de Investigación y Aplicación de Fitoquímicos Bioactivos, Posgrado en Botánica, Colegio de Postgraduados, Texcoco, 56230, Mexico. Electronic address: angel_jb_18@hotmail.com., Díaz-Nuñez JL; Laboratorio de Investigación y Aplicación de Fitoquímicos Bioactivos, Posgrado en Botánica, Colegio de Postgraduados, Texcoco, 56230, Mexico. Electronic address: diaz.jose@colpos.mx., Rivera-Chávez JA; Departamento de Productos Naturales, Instituto de Química, Universidad Nacional Autónoma de México, CDMX, Mexico. Electronic address: jrivera@iquimica.unam.mx., García-Contreras R; Departamento de Microbiología y Parasitología, Universidad Nacional Autónoma de México, Ciudad Universitaria, CDMX, 04510, Mexico. Electronic address: rgarc@bq.unam.mx., Estrada-Velasco ÁY; Departamento de Microbiología y Parasitología, Universidad Nacional Autónoma de México, Ciudad Universitaria, CDMX, 04510, Mexico. Electronic address: yahir.qbp2017@gmail.com., Quezada H; Laboratorio de Investigación en Inmunología y Proteómica, Hospital Infantil de México Federico Gómez, CDMX, 06720, Mexico. Electronic address: hquezada@himfg.edu.mx., Serrano Bello CA; Departamento de Patología Clínica y Experimental, Hospital Infantil de México Federico Gómez, CDMX, 06720, Mexico. Electronic address: crls.serrbe@gmail.com., Castillo-Juárez I; Laboratorio de Investigación y Aplicación de Fitoquímicos Bioactivos, Posgrado en Botánica, Colegio de Postgraduados, Texcoco, 56230, Mexico. Electronic address: israel.castillo@colpos.mx.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2024 Jan 30; Vol. 319 (Pt 2), pp. 117305. Date of Electronic Publication: 2023 Oct 15.
DOI: 10.1016/j.jep.2023.117305
Abstrakt: Ethnopharmacological Relevance: Cuachalalate (Amphipterygium adstringens) stem bark has been used to heal wounds and counteract microbial infections since pre-Hispanic times. However, its effect in treating infected burns remains unclear.
Study Objectives: To determine the antipathogenic capacity of a folk remedy (FR) containing cuachalalate stem bark to treat lesions caused by thermal damage and bacterial infection.
Materials and Methods: The antipathogenic capacity of the hexanic extract (HE) and FR was evaluated in a burned mouse model infected with Pseudomonas aeruginosa. Second to third-degree burns were induced with 95 °C water in CD1 mice in similar ratios of males to females. The mice were randomly grouped into non-inoculated (Group 1) and P. aeruginosa inoculated. The latter were divided into untreated infection (Group 2) and infection topically treated with HE (Group 3), silver sulfadiazine (Group 4), and tween 80 (Group 5). In the case of FR, the lesions were washed with an aqueous extract (AE) and applied powdered stem bark (Group 6). Animal survival, establishment of the bacteria in the lesions, and systemic dispersion were determined. In addition, histopathological analysis was performed. The chemical composition of the AE was analyzed through molecular networking analysis, and the antivirulence capacity was determined through the inhibition of pyocyanin production and caseinolytic activity.
Results: Only the FR showed antipathogenic activity and increased animal survival by 50% by reducing the systemic dispersion of P. aeruginosa. In addition, it stimulated the formation of granulation tissue and the generation of new blood vessels. The AE did not show bactericidal activity but reduced bacterial virulence, and glycosylated flavonoids and catechins were identified as its main constituents.
Conclusions: The results of this study contribute to validating the effectiveness of a popular remedy containing cuachalalate stem bark for treating burns infected with P. aeruginosa.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023. Published by Elsevier B.V.)
Databáze: MEDLINE
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