Effect of retrobulbar prostaglandin analog injection on orbital fat in rats.

Autor: Cosan S; Department of Ophthalmology, Faculty of Medicine, Hacettepe University, 06230, Sıhhiye, Ankara, Turkey., İnam O; Department of Biophysics, Faculty of Medicine, Gazi University, Ankara, Turkey., Kaya B; Department of Histology and Embryology, Faculty of Medicine, Hacettepe University, Ankara, Turkey., Atilla P; Department of Histology and Embryology, Faculty of Medicine, Hacettepe University, Ankara, Turkey., Ocal O; Department of Radiology, Hacettepe University, Ankara, Turkey., Arat A; Department of Radiology, Hacettepe University, Ankara, Turkey., Karakaya J; Department of Biostatistics, Hacettepe University, Ankara, Turkey., Arat YO; Department of Ophthalmology, Faculty of Medicine, Hacettepe University, 06230, Sıhhiye, Ankara, Turkey. yoncaozkan@hotmail.com.
Jazyk: angličtina
Zdroj: International ophthalmology [Int Ophthalmol] 2023 Dec; Vol. 43 (12), pp. 4985-4990. Date of Electronic Publication: 2023 Oct 17.
DOI: 10.1007/s10792-023-02901-0
Abstrakt: Purpose: Periorbital fat atrophy is a known side effect of topical prostaglandin analogs (PA). This side effect may have implications in the treatment of diseases like thyroid orbitopathy. In this in vivo study we aimed to evaluate the effects of retrobulbar injection of three different PAs on orbital fat.
Methods: Eighteen adult male Wistar-albino rats were divided into three groups of six animals. 0.1 ml of 0.03% bimatoprost, 0.005% latanoprost, or 0.005% travoprost was injected into the right orbits and saline was injected into the left orbits, as controls. Both orbits were exenterated after 3 weeks. Histological cross-sections were analyzed using ImageJ image analysis software. Intraconal adipocyte density was calculated.
Results: There was no significant difference in the adipocyte density between the PA injected orbits and the control side in each of the three groups. When calculations from all three groups were analyzed together, again the difference in the adipocyte density between the PA injected orbits and the control side was not significant.
Conclusion: No significant fat atrophy was noted in this rat model three weeks after retrobulbar injection of PAs. To evaluate retrobulbar injection of PA as a potential therapy for orbital diseases with fat proliferation, in vivo studies in different animal models, higher concentrations of PA, or longer follow-up duration are required.
(© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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