[Platelet-rich Plasma Induces M2 Macrophage Polarization via Regulating AMPK Singling Pathway].

Autor:	Shi LY; Department of Blood Transfusion, General Hospital of Southern Theatre Command of PLA, Guangzhou 510010, Guangdong Province, China., Li YH; Department of Blood Transfusion, General Hospital of Southern Theatre Command of PLA, Guangzhou 510010, Guangdong Province, China., Xu JJ; Department of Blood Transfusion, General Hospital of Southern Theatre Command of PLA, Guangzhou 510010, Guangdong Province, China., Zhang Y; Department of Blood Transfusion, General Hospital of Southern Theatre Command of PLA, Guangzhou 510010, Guangdong Province, China., Xie TT; Department of Blood Transfusion, General Hospital of Southern Theatre Command of PLA, Guangzhou 510010, Guangdong Province, China., Xu YB; Department of Blood Transfusion, General Hospital of Southern Theatre Command of PLA, Guangzhou 510010, Guangdong Province, China., Shan GQ; Department of Blood Transfusion, General Hospital of Southern Theatre Command of PLA, Guangzhou 510010, Guangdong Province, China.E-mail: rabbit_2007@126.com., Zhou M; Department of Blood Transfusion, General Hospital of Southern Theatre Command of PLA, Guangzhou 510010, Guangdong Province, China.E-mail: zhou_mou@163.com.
Jazyk:	čínština
Zdroj:	Zhongguo shi yan xue ye xue za zhi [Zhongguo Shi Yan Xue Ye Xue Za Zhi] 2023 Oct; Vol. 31 (5), pp. 1486-1491.
DOI:	10.19746/j.cnki.issn.1009-2137.2023.05.037
Abstrakt:	Objective: To investigate the role of platelet-rich plasma (PRP) in inducing the M2 macrophage polarization via regulating AMPK singling pathway. Methods: The expressions of M1 marker CD11c and M2 marker CD206 in macrophages of blank control group, LPS group, LPS+PRP group, and LPS+PRP+Compound C group were detected by flow cytometry. Western blot was used to observe the effects of PRP on the expression of AMPK-mTOR signaling pathway-related proteins at different times (12 h, 18 h and 24 h) after LPS treatment. RNA interference technology was used to silence the expression of AMPK in macrophages, and the expression of TGF-β protein was subsequently examined by Western blot. Results: LPS significantly reduced the expression of CD206 and increased the expression of CD11c ( P <0.05). After the addition of PRP, the expression of CD206 was significantly increased ( P <0.05), while the expression of CD11c was significantly decreased ( P <0.05). Compared with LPS group, PRP treatment significantly increased the expressions of p-AMPK and p-ULK1 proteins at 12 h, 18 h and 24 h, while significantly decreased the expression of p-mTOR protein ( P <0.05). After the addition of AMPK inhibitor Compound C, the expression of CD206 was significantly reduced ( P <0.05) and the expression of CD11c was significantly increased compared with LPS+PRP group ( P <0.05). After silencing the expression of AMPK in macrophages, the promotion effect of PRP on TGF-β was significantly reduced ( P <0.05). Conclusion: PRP can stimulate the transformation of macrophages to M2 type via AMPK signalling pathway.
Databáze:	MEDLINE
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