Methods to mitigate Escherichia coli blooms in human ex vivo colon model experiments using the high throughput micro-Matrix bioreactor fermentation system.

Autor:	Mathur H; Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland.; Food for Health Ireland, Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland., Mechoud MA; Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland.; Food for Health Ireland, Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland., Matthews C; Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland.; Food for Health Ireland, Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland., Lordan C; Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland.; Food for Health Ireland, Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland., FitzGerald JA; Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland.; Food for Health Ireland, Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland., Beresford T; Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland.; Food for Health Ireland, Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland., Cotter PD; Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland.; Food for Health Ireland, Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland.; APC Microbiome Ireland, University College Cork, Cork, Ireland.
Jazyk:	angličtina
Zdroj:	MethodsX [MethodsX] 2023 Oct 06; Vol. 11, pp. 102393. Date of Electronic Publication: 2023 Oct 06 (Print Publication: 2023).
DOI:	10.1016/j.mex.2023.102393
Abstrakt:	Ex vivo colon model experiments are frequently employed as a means to assess the gut microbiome modulating potential of different foods, food ingredients and dietary supplements. A number of useful models already exist; however, they tend to be relatively low in terms of throughput (3-4 samples per experiment) with a long experiment duration of one to a number of weeks. Therefore, a need for a high-throughput system with a short duration time is required to enable screening of large numbers of samples. Therefore, we report here on the development of a system based on the Applikon micro-Matrix bioreactor which has the capacity to run 24 samples with an experiment duration of 48 h. However, Escherichia coli blooms are a common problem encountered in this model. Here, we describe the factors that contribute to such blooms and provide approaches to address them, providing:•Step by step optimisation of processes involved in conducting ex vivo distal colon experiments using the micro-Matrix bioreactor fermentation platform•Recommended steps for users on how to attenuate E. coli blooms in such ex vivo colon model experiments. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2023 The Author(s).)
Databáze:	MEDLINE
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