Fluorescence lifetime of injected indocyanine green as a universal marker of solid tumours in patients.

Autor: Pal R; Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA., Lwin TM; Department of Surgical Oncology, City of Hope Hospital, Duarte, CA, USA., Krishnamoorthy M; Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA., Collins HR; Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA., Chan CD; Newcastle University Centre for Cancer, Translational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne, UK., Prilutskiy A; Department of Pathology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA., Nasrallah MP; Department of Pathology, Hospital of the University of Pennsylvania, Philadelphia, PA, USA., Dijkhuis TH; Department of Surgery, Leiden University Medical Center, Leiden, the Netherlands., Shukla S; Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA., Kendall AL; Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA., Marshall MS; Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA., Carp SA; Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA., Hung YP; Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA., Shih AR; Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA., Martinez-Lage M; Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA., Zukerberg L; Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA., Sadow PM; Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.; Department of Otolaryngology and Head and Neck Surgery, Massachusetts Eye and Ear, Harvard Medical School, Boston, MA, USA., Faquin WC; Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.; Department of Otolaryngology and Head and Neck Surgery, Massachusetts Eye and Ear, Harvard Medical School, Boston, MA, USA., Nahed BV; Department of Neurosurgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA., Feng AL; Department of Otolaryngology and Head and Neck Surgery, Massachusetts Eye and Ear, Harvard Medical School, Boston, MA, USA., Emerick KS; Department of Otolaryngology and Head and Neck Surgery, Massachusetts Eye and Ear, Harvard Medical School, Boston, MA, USA., Mieog JSD; Department of Surgery, Leiden University Medical Center, Leiden, the Netherlands., Vahrmeijer AL; Department of Surgery, Leiden University Medical Center, Leiden, the Netherlands., Rajasekaran K; Department of Otorhinolaryngology-Head and Neck Surgery, University of Pennsylvania, Philadelphia, PA, USA., Lee JYK; Department of Neurosurgery, Hospital of the University of Pennsylvania, Philadelphia, PA, USA., Rankin KS; The North of England Bone and Soft Tissue Tumour Service, Newcastle upon Tyne Hospitals NHS Foundation Trust, Newcastle upon Tyne, UK., Lozano-Calderon S; Department of Orthopedic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA., Varvares MA; Department of Otolaryngology and Head and Neck Surgery, Massachusetts Eye and Ear, Harvard Medical School, Boston, MA, USA., Tanabe KK; Division of Gastrointestinal and Oncologic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA., Kumar ATN; Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA. atkumar@mgh.harvard.edu.
Jazyk: angličtina
Zdroj: Nature biomedical engineering [Nat Biomed Eng] 2023 Dec; Vol. 7 (12), pp. 1649-1666. Date of Electronic Publication: 2023 Oct 16.
DOI: 10.1038/s41551-023-01105-2
Abstrakt: The surgical resection of solid tumours can be enhanced by fluorescence-guided imaging. However, variable tumour uptake and incomplete clearance of fluorescent dyes reduces the accuracy of distinguishing tumour from normal tissue via conventional fluorescence intensity-based imaging. Here we show that, after systemic injection of the near-infrared dye indocyanine green in patients with various types of solid tumour, the fluorescence lifetime (FLT) of tumour tissue is longer than the FLT of non-cancerous tissue. This tumour-specific shift in FLT can be used to distinguish tumours from normal tissue with an accuracy of over 97% across tumour types, and can be visualized at the cellular level using microscopy and in larger specimens through wide-field imaging. Unlike fluorescence intensity, which depends on imaging-system parameters, tissue depth and the amount of dye taken up by tumours, FLT is a photophysical property that is largely independent of these factors. FLT imaging with indocyanine green may improve the accuracy of cancer surgeries.
(© 2023. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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