The 5-azacytidine-induced epimutagenesis of sugarcane (Saccharum spp. hybrids) for aluminium tolerance.

Autor: Koetle MJ; South African Sugarcane Research Institute, Private Bag X02, Mount Edgecombe, Durban 4300, South Africa. Electronic address: KoetleM@arc.agric.za., Osborn C; Department of Molecular and Cell Biology, University of Cape Town, Rondebosch 7701, South Africa., Snyman SJ; South African Sugarcane Research Institute, Private Bag X02, Mount Edgecombe, Durban 4300, South Africa; School of Life Sciences, University of KwaZulu-Natal, Private Bag X54001, Durban 4300, South Africa., Rutherford RS; South African Sugarcane Research Institute, Private Bag X02, Mount Edgecombe, Durban 4300, South Africa; School of Life Sciences, University of KwaZulu-Natal, Private Bag X54001, Durban 4300, South Africa.
Jazyk: angličtina
Zdroj: Biochimica et biophysica acta. General subjects [Biochim Biophys Acta Gen Subj] 2023 Dec; Vol. 1867 (12), pp. 130491. Date of Electronic Publication: 2023 Oct 14.
DOI: 10.1016/j.bbagen.2023.130491
Abstrakt: A protocol for generating aluminium (Al) tolerant sugarcane using 5-azacytidine (5-azaC)-induced epimutagenesis was developed. Eight (8) plantlets per gram of cultivar N51 calli were generated from the 5-azaC (100 μM) and Al (1.5-50 mM) treatment (+Az + Al) when compared with 104 plantlets from non-mutagenised, no Al control treatment. Following in vitro selection on 60 mM Al, ex vitro stress over four rounds of chimera dissolution during vegetative propagation was applied. In the final stress round, 36% of the Mut 2 epilines survived with plants showing higher relative water content (61.2%) when compared with 47.3% from the stressed control (S N51). The Mut 2 line maintained a higher green leaf area (83.4%) and longer roots (32.4 cm) under stress than S N51 (61.4% and 26.3 cm, respectively). Overall, Mut 2 had a high stress tolerance index of 85.4%, compared with 79.0% from the S N51, nearing that of the non-stressed N51 control (NS N51, 100%) when data were analysed using PCA and clustering analyses of morpho-physiological traits. Analysis of molecular variance (AMOVA) revealed high epigenetic differentiation (ɸst = 0.67) and a variation of 66.6% observed among N51 genotypes. The principal coordinate analysis (PCoA) showed that Mut 2 was epigenetically distinct from S N51. These findings support previous studies that 5-azaC can be used for novel trait creation via epimutagenesis and highlights the necessity for chimera dissolution to achieve stable traits in epibreeding of sugarcane.
Competing Interests: Declaration of Competing Interest The authors declare no competing and conflicting interests. No AI sources were used during the compilation of this manuscript. The authors further confirm that the data supporting the findings of this study are available within the article. Raw data were generated at the South African Sugarcane Research Institute and can be made available upon request.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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