Triphasic production of IFN γ by innate and adaptive lymphocytes following influenza A virus infection.

Autor:	Finney GE; Centre for Immunobiology, School of Infection and Immunity, University of Glasgow, Glasgow, UK., Hargrave KE; Centre for Immunobiology, School of Infection and Immunity, University of Glasgow, Glasgow, UK., Pingen M; Centre for Immunobiology, School of Infection and Immunity, University of Glasgow, Glasgow, UK., Purnell T; Centre for Immunobiology, School of Infection and Immunity, University of Glasgow, Glasgow, UK., Todd D; Centre for Immunobiology, School of Infection and Immunity, University of Glasgow, Glasgow, UK., MacDonald F; Centre for Immunobiology, School of Infection and Immunity, University of Glasgow, Glasgow, UK., Worrell JC; Centre for Immunobiology, School of Infection and Immunity, University of Glasgow, Glasgow, UK., MacLeod MKL; Centre for Immunobiology, School of Infection and Immunity, University of Glasgow, Glasgow, UK.
Jazyk:	angličtina
Zdroj:	Discovery immunology [Discov Immunol] 2023 Aug 19; Vol. 2 (1), pp. kyad014. Date of Electronic Publication: 2023 Aug 19 (Print Publication: 2023).
DOI:	10.1093/discim/kyad014
Abstrakt:	Interferon gamma (IFN γ ) is a potent antiviral cytokine that can be produced by many innate and adaptive immune cells during infection. Currently, our understanding of which cells produce IFN γ and where they are located at different stages of an infection is limited. We have used reporter mice to investigate in vivo expression of Ifn γ mRNA in the lung and secondary lymphoid organs during and following influenza A virus (IAV) infection. We observed a triphasic production of Ifn γ expression. Unconventional T cells and innate lymphoid cells, particularly NK cells, were the dominant producers of early Ifn γ , while CD4 and CD8 T cells were the main producers by day 10 post-infection. Following viral clearance, some memory CD4 and CD8 T cells continued to express Ifn γ in the lungs and draining lymph node. Interestingly, Ifn γ production by lymph node natural killer (NK), NKT, and innate lymphoid type 1 cells also continued to be above naïve levels, suggesting memory-like phenotypes for these cells. Analysis of the localization of Ifn γ + memory CD4 and CD8 T cells demonstrated that cytokine+ T cells were located near airways and in the lung parenchyma. Following a second IAV challenge, lung IAV-specific CD8 T cells rapidly increased their expression of Ifn γ while CD4 T cells in the draining lymph node increased their Ifn γ response. Together, these data suggest that Ifn γ production fluctuates based on cellular source and location, both of which could impact subsequent immune responses. Competing Interests: None declared. (© The Author(s) 2023. Published by Oxford University Press on behalf of the British Society for Immunology.)
Databáze:	MEDLINE
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