Fine mapping of the major gene BhHLS1 controlling seed size in wax gourd ( Benincasa hispida ).

Autor: Yang W; College of Agriculture, Guangxi University, Nanning, China., Wang P; College of Agriculture, Guangxi University, Nanning, China., Liu T; College of Agriculture, Guangxi University, Nanning, China., Nong L; College of Agriculture, Guangxi University, Nanning, China., Cheng Z; College of Agriculture, Guangxi University, Nanning, China., Su L; College of Agriculture, Guangxi University, Nanning, China., Bai W; College of Agriculture, Guangxi University, Nanning, China., Deng Y; College of Agriculture, Guangxi University, Nanning, China., Chen Z; College of Agriculture, Guangxi University, Nanning, China., Liu Z; College of Agriculture, Guangxi University, Nanning, China.
Jazyk: angličtina
Zdroj: Frontiers in plant science [Front Plant Sci] 2023 Sep 29; Vol. 14, pp. 1266796. Date of Electronic Publication: 2023 Sep 29 (Print Publication: 2023).
DOI: 10.3389/fpls.2023.1266796
Abstrakt: Introduction/background: The seed size of wax gourds is an important agronomic trait; however, the associated genes have not yet been reported.
Methods: In this study, we used a high-density genetic map constructed based on F 8 recombinant inbred line populations derived from a cross between MY-1 (large seed) and GX-71 (small seed) strains to detect quantitative trait locis (QTLs) for seed-size-related traits in wax gourd over a two-year period.
Results: Two stable QTLs ( qSL10 and qSW10 ) for seed length (SL) and seed width (SW) on chromosome 10 were repeatedly detected over two years (2021-2022). qSL10 had a phenotypic variation rate of 75.30% and 80.80% in 2021 and 2022, respectively. Whereas, qSW10 had a phenotypic variation rate of 66.60% and 73.80% in 2021 and 2022, respectively. Further, a single nucleotide polymorphism mutation was found to cause early termination of Bch10G006400 ( BhHLS1 ) translation in GX-71 through sequencing analysis of candidate genes. Based on gene functional annotation and quantitative real-time PCR analyses, BhHLS1 encoded a probable N-acetyltransferase HLS1-like protein and its expression level was significantly different between parents. Therefore, BhHLS1 is a major candidate gene associated with a one-factor polymorphism regulating the SL and SW of wax gourds. Finally, based on variation in the BhHLS1 sequence, a cleaved amplified polymorphic sequence marker was developed for the molecular marker-assisted breeding of wax gourds.
Discussion: Overall, this study is of great significance for the genetic improvement of seed size, verification of gene functions, and cultivation of specific germplasm resources for wax gourds.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 Yang, Wang, Liu, Nong, Cheng, Su, Bai, Deng, Chen and Liu.)
Databáze: MEDLINE
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