Analysis of the milk kefir pan-metagenome reveals four community types, core species, and associated metabolic pathways.

Autor:	Walsh LH; Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland.; School of Microbiology, University College Cork, Ireland., Coakley M; Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland., Walsh AM; Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland., Crispie F; Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland., O'Toole PW; School of Microbiology, University College Cork, Ireland.; APC Microbiome Ireland SFI Research Centre, University College Cork, Ireland., Cotter PD; Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland.; APC Microbiome Ireland SFI Research Centre, University College Cork, Ireland.; VistaMilk SFI Research Centre, Teagasc, Moorepark, Fermoy, Co. Cork, Ireland.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2023 Sep 21; Vol. 26 (10), pp. 108004. Date of Electronic Publication: 2023 Sep 21 (Print Publication: 2023).
DOI:	10.1016/j.isci.2023.108004
Abstrakt:	A comprehensive metagenomics-based investigation of the microorganisms present within milk kefir communities from across the globe was carried out with a view to defining the milk kefir pan-metagenome, including details relating to core and non-core components. Milk kefir samples, generated by inoculating full fat, pasteurized cow's milk with 64 kefir grains sourced from 25 different countries, were analyzed. We identified core features, including a consistent pattern of domination by representatives from the species Lactobacillus helveticus or the sub-species Lactobacillus kefiranofaciens subsp . kefiranofaciens , Lactococcus lactis subsp . lactis or Lla. cremoris subsp . cremoris in each kefir. Notably, even in kefirs where the lactococci did not dominate, they and 51 associated metabolic pathways were identified across all metagenomes. These insights can contribute to future efforts to create tailored kefir-based microbial communities for different applications and assist regulators and producers to ensure that kefir products have a microbial composition that reflects the artisanal beverage. Competing Interests: The authors declare no conflicting interest. (© 2023 The Author(s).)
Databáze:	MEDLINE
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