Producing Tailocins from Phages Using Osmotic Shock and Benzalkonium Chloride.
| Autor: | Woudstra C; Department of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg C, Denmark., Brøndsted L; Department of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg C, Denmark. |
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| Jazyk: | angličtina |
| Zdroj: | PHAGE (New Rochelle, N.Y.) [Phage (New Rochelle)] 2023 Sep 01; Vol. 4 (3), pp. 136-140. Date of Electronic Publication: 2023 Sep 20. |
| DOI: | 10.1089/phage.2023.0014 |
| Abstrakt: | In the light of the worldwide antimicrobial resistance crisis, new substitutes to antibiotics are urgently needed. Tailocins or phage tail-like bacteriocin particles, produced by bacteria for environmental competition, are a potential antimicrobial alternative to antibiotic treatment. Yet, the availability of characterized Tailocins is limited. We explored the possibility to produce new Tailocins from phage particles, using osmotic shock or chemical treatment by the ammonium quaternary compound benzalkonium chloride on Ackermannviridae phage S117 and using Straboviridae phage T4 as control. We report that phage S117 was resistant to such treatment, while successful production of Tailocins by osmotic shock was achieved for phage T4. Finally, chemical treatment with benzalkonium chloride was inefficient on phage S117 but successfully inactivated phage T4 without production of Tailocins. Further studies are needed to implement such treatments of phages for producing Tailocins with killing activity. Competing Interests: No potential conflict of interest was reported by the authors. (Copyright 2023, Mary Ann Liebert, Inc., publishers.) |
| Databáze: | MEDLINE |
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