X-ray phase-contrast microtomography of soft tissues using a compact laboratory system with two-directional sensitivity.

Autor:	Navarrete-León C; Department of Medical Physics and Biomedical Engineering, University College London, Gower Street, London, WC1E 6BT, UK., Doherty A; Department of Medical Physics and Biomedical Engineering, University College London, Gower Street, London, WC1E 6BT, UK., Savvidis S; Department of Medical Physics and Biomedical Engineering, University College London, Gower Street, London, WC1E 6BT, UK., Gerli MFM; UCL Division of Surgery and Interventional Science, Royal Free Hospital, Rowland Hill Street, London, NW3 2PF, UK.; Stem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College London, London, WC1N 1EH, UK., Piredda G; Research Center for Microtechnology, Vorarlberg University of Applied Sciences, Hochschulstr. 1, 6850, Dornbirn, Austria., Astolfo A; Department of Medical Physics and Biomedical Engineering, University College London, Gower Street, London, WC1E 6BT, UK., Bate D; Department of Medical Physics and Biomedical Engineering, University College London, Gower Street, London, WC1E 6BT, UK.; Nikon X-Tek Systems Ltd, Tring, Herts, HP23 4JX, UK., Cipiccia S; Department of Medical Physics and Biomedical Engineering, University College London, Gower Street, London, WC1E 6BT, UK., Hagen CK; Department of Medical Physics and Biomedical Engineering, University College London, Gower Street, London, WC1E 6BT, UK., Olivo A; Department of Medical Physics and Biomedical Engineering, University College London, Gower Street, London, WC1E 6BT, UK., Endrizzi M; Department of Medical Physics and Biomedical Engineering, University College London, Gower Street, London, WC1E 6BT, UK.
Jazyk:	angličtina
Zdroj:	Optica [Optica] 2023 Jun 30; Vol. 10 (7), pp. 880-887. Date of Electronic Publication: 2023 Jun 30 (Print Publication: 2023).
DOI:	10.1364/OPTICA.487270
Abstrakt:	X-ray microtomography is a nondestructive, three-dimensional inspection technique applied across a vast range of fields and disciplines, ranging from research to industrial, encompassing engineering, biology, and medical research. Phase-contrast imaging extends the domain of application of x-ray microtomography to classes of samples that exhibit weak attenuation, thus appearing with poor contrast in standard x-ray imaging. Notable examples are low-atomic-number materials, like carbon-fiber composites, soft matter, and biological soft tissues. We report on a compact and cost-effective system for x-ray phase-contrast microtomography. The system features high sensitivity to phase gradients and high resolution, requires a low-power sealed x-ray tube, a single optical element, and fits in a small footprint. It is compatible with standard x-ray detector technologies: in our experiments, we have observed that single-photon counting offered higher angular sensitivity, whereas flat panels provided a larger field of view. The system is benchmarked against known-material phantoms, and its potential for soft-tissue three-dimensional imaging is demonstrated on small-animal organs: a piglet esophagus and a rat heart. We believe that the simplicity of the setup we are proposing, combined with its robustness and sensitivity, will facilitate accessing quantitative x-ray phase-contrast microtomography as a research tool across disciplines, including tissue engineering, materials science, and nondestructive testing in general. Competing Interests: The authors declare no conflicts of interest. (Published by Optica Publishing Group under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.)
Databáze:	MEDLINE
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