CRISPR-based Biosensors for Human Health: A Novel Strategy to Detect Emerging Infectious Diseases.

Autor: He Y; Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA 24061, USA., Hu Q; School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.; One Health Center, Shanghai Jiao Tong University-The University of Edinburgh, Shanghai, People's Republic of China., San S; Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA 24061, USA., Kasputis T; Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA 24061, USA., Splinter MGD; Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA 24061, USA., Yin K; School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.; One Health Center, Shanghai Jiao Tong University-The University of Edinburgh, Shanghai, People's Republic of China., Chen J; Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA 24061, USA.
Jazyk: angličtina
Zdroj: Trends in analytical chemistry : TRAC [Trends Analyt Chem] 2023 Nov; Vol. 168. Date of Electronic Publication: 2023 Sep 30.
DOI: 10.1016/j.trac.2023.117342
Abstrakt: Infectious diseases (such as sepsis, influenza, and malaria), caused by various pathogenic bacteria and viruses, are widespread across the world. Early and rapid detection of disease-related pathogens is necessary to reduce their spread in the world and prevent their potential global pandemics. The clustered regularly interspaced short palindromic repeats (CRISPR) technology, as the next-generation molecular diagnosis technique, holds immense promise in the detection of infectious diseases because of its remarkable advantages, including supreme flexibility, sensitivity, and specificity. While numerous CRISPR-based biosensors have been developed for application in environmental monitoring, food safety, and point-of-care diagnosis, there remains a critical need to summarize and explore their potential in human health. This review aims to address this gap by focusing on the latest advancements in CRISPR-based biosensors for infectious disease detection. We provide an overview of the current status, pre-amplification methods, the unique feature of each CRISPR system, and the design of CRISPR-based biosensing strategies to detect disease-associated nucleic acids. Last but not least, the review analyzes the current challenges and provides future perspectives, which will contribute to developing more effective CRISPR-based biosensors for human health.
Competing Interests: Declaration of interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Databáze: MEDLINE
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