GhL1L1 regulates the contents of unsaturated fatty acids by activating the expression of GhFAD2 genes in cotton.

Autor: Yue D; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China. Electronic address: ddyue@webmail.hzau.edu.cn., Hao X; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China. Electronic address: Haoxuy@webmail.hzau.edu.cn., Han B; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China. Electronic address: bhan@webmail.hzau.edu.cn., Xu J; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China; Resource Institute for Chinese Medicine and Ethnic Materia Medica, Guizhou University of Traditional Chinese Medicine, Guiyang 550000, Guizhou, China. Electronic address: xujiao2008mzk@163.com., Sun W; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China. Electronic address: wnsun@webmail.hzau.edu.cn., Guo X; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China. Electronic address: xpguo@mail.hzau.edu.cn., Zhang X; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China. Electronic address: xlzhang@mail.hzau.edu.cn., Yang X; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China. Electronic address: yxy@mail.hzau.edu.cn.
Jazyk: angličtina
Zdroj: Gene [Gene] 2024 Jan 30; Vol. 893, pp. 147899. Date of Electronic Publication: 2023 Oct 13.
DOI: 10.1016/j.gene.2023.147899
Abstrakt: Edible oils with high unsaturated fatty acids, particularly oleic acid, are beneficial to human health. Cotton is one of the top five oil crops in the world, but the mechanism of high-quality oil synthesis and regulatory networks in cotton are largely unclear. Here, we identified Leafy cotyledon1-like 1 (GhL1L1), a NF-YB subfamily gene that is specifically expressed during somatic embryogenesis and seed maturation in cotton. Overexpression of GhL1L1 regulates the contents of unsaturated fatty acids in cotton, especially in the seeds, which is associated with altered expression of the cotton fatty acid biosynthesis-related genes. GhL1L1 synergistically enhanced the expression of GhFAD2-1A by binding to the G-box in its promoter, leading to an increase in the content of linoleic acid. Furthermore, this activation could be enhanced by GhNF-YC2 and GhNF-YA1 by form a transcriptional complex. Collectively, these results contribute to provide new insights into the molecular mechanism of oil biosynthesis in cotton and can facilitate genetic manipulation of cotton varieties with enhanced oil content.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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