Molecular detection and associated risk factors of Anaplasma marginale, A. ovis and A. platys in sheep from Algeria with evidence of the absence of A. phagocytophilum.

Autor: Chadi H; Research Laboratory Management of Local Animal Resources, Higher National Veterinary School (ENSV), El Harrach, Algiers 16004, Algeria; Department of Applied Biology, Faculty of Exact Sciences and Nature and Life Sciences, University of Echahid Cheikh Larbi Tebessi, Tebessa 12000, Algeria. Electronic address: hafidha.chadi@univ-tebessa.dz., Moraga-Fernández A; SaBio, Instituto de Investigación en Recursos Cinegéticos (IREC), Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Castilla-La Mancha (UCLM)-Junta de Comunidades de Castilla-La Mancha (JCCM), Ronda de Toledo 12, Ciudad Real 13005, Spain., Sánchez-Sánchez M; SaBio, Instituto de Investigación en Recursos Cinegéticos (IREC), Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Castilla-La Mancha (UCLM)-Junta de Comunidades de Castilla-La Mancha (JCCM), Ronda de Toledo 12, Ciudad Real 13005, Spain., Chenchouni H; Higher National School of Forests, Khenchela 40000, Algeria., Fernández de Mera IG; SaBio, Instituto de Investigación en Recursos Cinegéticos (IREC), Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Castilla-La Mancha (UCLM)-Junta de Comunidades de Castilla-La Mancha (JCCM), Ronda de Toledo 12, Ciudad Real 13005, Spain., Garigliany MM; Fundamental and Applied Research for Animals and Health (FARAH), Faculty of Veterinary Medicine, University of Liège, Belgium., de la Fuente J; SaBio, Instituto de Investigación en Recursos Cinegéticos (IREC), Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Castilla-La Mancha (UCLM)-Junta de Comunidades de Castilla-La Mancha (JCCM), Ronda de Toledo 12, Ciudad Real 13005, Spain; Center for Veterinary Health Sciences, Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK 74078, USA., Tennah S; Research Laboratory Management of Local Animal Resources, Higher National Veterinary School (ENSV), El Harrach, Algiers 16004, Algeria., Sedrati T; Research Laboratory Management of Local Animal Resources, Higher National Veterinary School (ENSV), El Harrach, Algiers 16004, Algeria; Department of Biology, University of Mohamed El Bachir El Ibrahimi, Bordj Bou Arréridj 34000, Algeria., Ghalmi F; Research Laboratory Management of Local Animal Resources, Higher National Veterinary School (ENSV), El Harrach, Algiers 16004, Algeria.
Jazyk: angličtina
Zdroj: Acta tropica [Acta Trop] 2024 Jan; Vol. 249, pp. 107040. Date of Electronic Publication: 2023 Oct 13.
DOI: 10.1016/j.actatropica.2023.107040
Abstrakt: Anaplasma species are obligate intracellular rickettsial pathogens that cause significant diseases in animals and humans. Despite their importance, limited information on Anaplasma infections in Algeria has been published thus far. This study aimed to assess the infection rate, characterize Anaplasma species, and identify associated risk factors in selected sheep farms across Oum El Bouaghi region in Algeria. In 2018, we collected 417 blood samples from sheep (Ovis aries) and performed molecular characterization of Anaplasma species infecting these animals. This characterization involved the use of 16S rRNA, msp2, rpoB, and msp5 genes, which were analyzed through nested PCR, qPCR, cPCR, DNA sequencing, and subsequent phylogenetic analysis. Our findings revealed infection rates of 12.7 % for Anaplasma species detected, with Anaplasma ovis at 10.8 %, Anaplasma marginale at 1.7 %, and Anaplasma platys at 0.2 %. Interestingly, all tested animals were found negative for Anaplasma phagocytophilum. Statistical analyses, including the Chi-square test and Fisher exact test, failed to establish any significant relationships (p > 0.05) between A. ovis and A. platys infections and variables such as age, sex, sampling season, and tick infestation level. However, A. marginale infection exhibited a significant association with age (p < 0.05), with a higher incidence observed in lambs (5.2 %) compared to other age groups. Remarkably, this study represents the first molecular detection of A. platys and A. marginale in Algerian sheep. These findings suggest that Algerian sheep may serve as potential reservoirs for these pathogens. This research contributes valuable insights into the prevalence and characteristics of Anaplasma infections in Algerian sheep populations, emphasizing the need for further investigation and enhanced surveillance to better understand and manage these diseases.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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