Trypanosomatids in honey bee colonies in Spain: A new specific qPCR method for specific quantification of Lotmaria passim, Crithidia mellificae and Crithidia bombi.

Autor: Buendía-Abad M; Laboratorio de Patología Apícola, Centro de Investigación Apícola y Agroambiental (CIAPA), IRIAF - Instituto de Investigación y Desarrollo Agroalimentario y Forestal, Consejería de Agricultura, Agua y Desarrollo Rural de la Junta de Comunidades de Castilla-La Mancha, 19180 Marchamalo, Spain. Electronic address: mbuendia92@gmail.com., Martín-Hernández R; Laboratorio de Patología Apícola, Centro de Investigación Apícola y Agroambiental (CIAPA), IRIAF - Instituto de Investigación y Desarrollo Agroalimentario y Forestal, Consejería de Agricultura, Agua y Desarrollo Rural de la Junta de Comunidades de Castilla-La Mancha, 19180 Marchamalo, Spain; Instituto de Recursos Humanos para la Ciencia y la Tecnología (Increcyt-Feder), Fundación Parque Científico y Tecnológico de Castilla-La Mancha, 02001 Albacete, Spain. Electronic address: rmhernandez@jccm.es., Higes M; Laboratorio de Patología Apícola, Centro de Investigación Apícola y Agroambiental (CIAPA), IRIAF - Instituto de Investigación y Desarrollo Agroalimentario y Forestal, Consejería de Agricultura, Agua y Desarrollo Rural de la Junta de Comunidades de Castilla-La Mancha, 19180 Marchamalo, Spain. Electronic address: mhiges@jccm.es.
Jazyk: angličtina
Zdroj: Journal of invertebrate pathology [J Invertebr Pathol] 2023 Nov; Vol. 201, pp. 108004. Date of Electronic Publication: 2023 Oct 13.
DOI: 10.1016/j.jip.2023.108004
Abstrakt: Bee trypanosomatids have not been widely studied due to the original belief that these organisms were not pathogenic to honey bees. However, trypanosomatids have been linked to increased winter mortality in honey bee colonies in recent years and it has been shown that these pathogens can shorten a honey bee worker's lifespan in laboratory conditions. These studies found that this mortality corresponded to dose-dependent infection. Although Lotmaria passim is the most prevalent species worldwide, the natural load in colonies remains poorly investigated. Here we describe a new highly specific and sensitive qPCR method that allows the differentiation and quantification of the parasitic load of each of the three most common trypanosomatid species described to date in honey bee colonies: L. passim, Crithidia mellificae, and Crithidia bombi. We have used this new method to analyze honey bee colonies in central Spain and confirm that L. passim is the most common species and the one with higher parasitic loads in the colonies, which increased over the years, being higher in spring than in autumn. Crithidia mellificae was present along the study, with the highest prevalence in autumn 2019 and lately it was only found in non-quantifiable loads. Crithidia bombi was not detected in any of the colonies analyzed.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023. Published by Elsevier Inc.)
Databáze: MEDLINE
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