The gain-of-function mutation blf13 in the barley orthologue of the rice growth regulator NARROW LEAF1 is associated with increased leaf width.

Autor:	Jöst M; Institute for Biochemistry and Biology, University of Potsdam, Potsdam, Germany., Soltani O; Institute for Biochemistry and Biology, University of Potsdam, Potsdam, Germany., Kappel C; Institute for Biochemistry and Biology, University of Potsdam, Potsdam, Germany., Janiak A; Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, Poland., Chmielewska B; Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, Poland., Szurman-Zubrzycka M; Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, Poland., McKim SM; Division of Plant Sciences, School of Life Sciences, University of Dundee, Dundee, UK., Lenhard M; Institute for Biochemistry and Biology, University of Potsdam, Potsdam, Germany.
Jazyk:	angličtina
Zdroj:	Journal of experimental botany [J Exp Bot] 2024 Feb 02; Vol. 75 (3), pp. 850-867.
DOI:	10.1093/jxb/erad403
Abstrakt:	Canopy architecture in cereals plays an important role in determining yield. Leaf width represents one key aspect of this canopy architecture. However, our understanding of leaf width control in cereals remains incomplete. Classical mutagenesis studies in barely identified multiple morphological mutants, including those with differing leaf widths. Of these, we characterized the broad leaf13 (blf13) mutant in detail. Mutant plants form wider leaves due to increased post-initiation growth and cell proliferation. The mutant phenotype perfectly co-segregated with a missense mutation in the HvHNT1 gene which affected a highly conserved region of the encoded protein, orthologous to the rice NARROW LEAF1 (NAL1) protein. Causality of this mutation for the blf13 phenotype is further supported by correlative transcriptomic analyses and protein-protein interaction studies showing that the mutant HvNHT1 protein interacts more strongly with a known interactor than wild-type HvHNT1. The mutant HvHNT1 protein also showed stronger homodimerization compared with wild-type HvHNT1, and homology modelling suggested an additional interaction site between HvHNT1 monomers due to the blf13 mutation. Thus, the blf13 mutation parallels known gain-of-function NAL1 alleles in rice that increase leaf width and grain yield, suggesting that the blf13 mutation may have a similar agronomic potential in barley. (© The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
Databáze:	MEDLINE
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