| Autor: |
Uddin MMN; School of Science, Western Sydney University, Penrith, NSW 2750, Australia.; Department of Pharmacy, Faculty of Biological Sciences, University of Chittagong, Chittagong 4331, Bangladesh., Bekmukhametova A; School of Science, Western Sydney University, Penrith, NSW 2750, Australia., Antony A; School of Science, Western Sydney University, Penrith, NSW 2750, Australia., Barman SK; School of Science, Western Sydney University, Penrith, NSW 2750, Australia., Houang J; School of Science, Western Sydney University, Penrith, NSW 2750, Australia., Wu MJ; School of Science, Western Sydney University, Penrith, NSW 2750, Australia., Hook J; School of Chemistry, University of New South Wales, Sydney, NSW 2052, Australia., George L; Advanced Materials Characterisation Facility, Western Sydney University, Penrith, NSW 2750, Australia., Wuhrer R; Advanced Materials Characterisation Facility, Western Sydney University, Penrith, NSW 2750, Australia., Mawad D; School of Materials Science and Engineering and Australian Centre for NanoMedicine, University of New South Wales, Kensington, NSW 2052, Australia., Ta D; School of Science, Western Sydney University, Penrith, NSW 2750, Australia., Lauto A; School of Science, Western Sydney University, Penrith, NSW 2750, Australia.; Biomedical Engineering & Neuroscience Research Group, The MARCS Institute, Western Sydney University, Penrith, NSW 2750, Australia. |
| Abstrakt: |
Cancer, a prominent cause of death, presents treatment challenges, including high dosage requirements, drug resistance, poor tumour penetration and systemic toxicity in traditional chemotherapy. Photodynamic therapy, using photosensitizers like rose bengal (RB) with a green laser, shows promise against breast cancer cells in vitro. However, the hydrophilic RB struggles to efficiently penetrate the tumour site due to the unique clinical microenvironment, aggregating around rather than entering cancer cells. In this study, we have synthesized and characterized RB-encapsulated chitosan nanoparticles with a peak particle size of ~200 nm. These nanoparticles are readily internalized by cells and, in combination with a green laser (λ = 532 nm) killed 94-98% of cultured human breast cancer cells (MCF-7) and prostate cancer cells (PC3) at a low dosage (25 μg/mL RB-nanoparticles, fluence ~126 J/cm 2 , and irradiance ~0.21 W/cm 2 ). Furthermore, these nanoparticles are not toxic to cultured human normal breast cells (MCF10A), which opens an avenue for translational applications. |
