| Autor: |
Uner B; Department of Pharmaceutical Technology, Institute of Graduate Studies in Health Sciences, Istanbul University, Istanbul, Turkey.; Department of Pharmaceutical Technology, Faculty of Pharmacy, Yeditepe University, Istanbul, Turkey.; Department of Pharmaceutical and Administrative Sciences, University of Health Science and Pharmacy in St. Louis, St. Louis, Missouri, USA., Durgun ME; Department of Pharmaceutical Technology, Faculty of Pharmacy, Istanbul University, Istanbul, Turkey., Ozdemir S; Department of Pharmaceutical Technology, Faculty of Pharmacy, Istanbul Health and Technology University, Istanbul, Turkey., Tas C; Department of Pharmaceutical Technology, Faculty of Pharmacy, Yeditepe University, Istanbul, Turkey., Uner M; Department of Pharmaceutical Technology, Faculty of Pharmacy, Istanbul University, Istanbul, Turkey., Ozsoy Y; Department of Pharmaceutical Technology, Faculty of Pharmacy, Istanbul University, Istanbul, Turkey. |
| Abstrakt: |
Ocular drug delivery methods are highly favored for boosting bioavailability, patient compliance, and lower adverse effects and dose frequency. In addition to preventing adverse effects from the active ingredient, the parts of drug delivery systems must be nontoxic and nonallergic as well. Mitochondrial toxicity test (MTT) and Hen's egg chorioallantois membrane (HET-CAM) assay are the most often utilized tests based on this dilemma. The toxicity of loteprednol etabonate loaded solid lipid nanoparticles, lipid nanostructured carriers, and nanoemulsion were compared. Oleic acid, Precirol ® ATO5, and Pluronic ® F68 were used in the preparation. Their toxicities were evaluated by using two different toxicity tests (MTT and HET-CAM). The results suggest that there are no significant differences between the HET-CAM and MTT assays. It is noteworthy that the HET-CAM assay offers a more cost-effective and environmentally friendly alternative to the MTT assay, as it does not require cell culture and generates less toxic waste. This information may be useful to consider when selecting between the two assays . |
