Long-term culture induces Bax-dependent apoptosis in rat preimplantation embryos.

Autor: Nakamura K; Advanced Medicine, Innovation and Clinical Research Center, Tottori University Hospital, Yonago, Tottori, Japan., Seno M; Advanced Medicine & Translational Research Center, Organization for Research Initiative and Promotion, Tottori University, Yonago, Tottori, Japan., Yoshimura Y; Division of Integrative Physiology, Faculty of Medicine, Tottori University, Yonago, Tottori, Japan., Suzuki O; Laboratory of Animal Models for Human Diseases, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki, Osaka, Japan.
Jazyk: angličtina
Zdroj: Molecular reproduction and development [Mol Reprod Dev] 2024 Jan; Vol. 91 (1), pp. e23711. Date of Electronic Publication: 2023 Oct 13.
DOI: 10.1002/mrd.23711
Abstrakt: Although rat preimplantation embryos are necessary for producing genetically modified rats, their in vitro culture remains a challenge. Rat zygotes can develop from the one-cell stage to the blastocyst stage in vitro; however, long-term culture reduces their developmental competence via an unknown mechanism. In this study, we examined how in vitro conditions affect rat preimplantation embryos, which may explain this reduced competence. Comprehensive gene expression analysis showed that genes related to apoptosis and energy metabolism were differentially expressed in rat embryos cultured long-term in vitro compared with those developed in vivo. Furthermore, we found that the expression of Bak1 and Bax, which are responsible for mitochondrial outer membrane permeabilization, were more upregulated in embryos cultured in vitro than those developed in vivo. Similarly, apoptosis-dependent DNA fragmentation was also exacerbated in in vitro culture conditions. Finally, gene disruption using CRISPR/Cas9 showed that Bax, but not Bak1, was responsible for these effects. These findings suggest that long-term in vitro culture induces Bax-dependent apoptosis through the mitochondrial pathway and may provide clues to improve the long-term culture of rat preimplantation embryos for genetic engineering research.
(© 2023 Wiley Periodicals LLC.)
Databáze: MEDLINE
Externí odkaz: