Protocol for generation of a time-resolved cellular interactome during tissue remodeling in adult mice.
| Autor: | Groppa E; Borea Therapeutics, Scuola Internazionale Superiore di Studi Avanzati, 34136 Trieste, Italy. Electronic address: egroppa@sissa.it., Tung LW; School of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 2B9, Canada., Mattevi S; Department of Molecular and Translational Medicine, University of Brescia, 25121 Brescia, Italy., Ritso M; School of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 2B9, Canada., Rossi FMV; School of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 2B9, Canada., Martini P; Department of Molecular and Translational Medicine, University of Brescia, 25121 Brescia, Italy. Electronic address: paolo.martini@unibs.it. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2023 Dec 15; Vol. 4 (4), pp. 102638. Date of Electronic Publication: 2023 Oct 11. |
| DOI: | 10.1016/j.xpro.2023.102638 |
| Abstrakt: | Efficient skeletal muscle regeneration necessitates fine-tuned coordination among multiple cell types through an intricate network of intercellular communication. We present a protocol for generation of a time-resolved cellular interactome during tissue remodeling. We describe steps for isolating distinct cell populations from skeletal muscle of adult mice after acute damage and extracting RNA from purified cells prior to the generation of RNA sequencing data. We then detail procedures for generating and deciphering a time- and lineage-resolved model of intercellular crosstalk. For complete details on the use and execution of this protocol, please refer to Groppa et al. (2023). 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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