Molecular Identification of Aedes, Armigeres, and Culex Mosquitoes (Diptera: Culicidae) Using Mitochondrial Cytochrome Oxidase Subunit I Genes in Myanmar.

Autor: Aung ST; Department of Pharmacology and Parasitology, University of Veterinary Science, Yezin, Nay Pyi Taw, 15013, Myanmar.; Swan Arh Veterinary Clinic, Yan Aung, Pyinmana, Nay Pyi Taw, 15012, Myanmar., Bawm S; Department of Pharmacology and Parasitology, University of Veterinary Science, Yezin, Nay Pyi Taw, 15013, Myanmar. sawvet@uvsyezin.edu.mm.; Department of Livestock and Aquaculture Research, Yezin, Nay Pyi Taw, 15013, Myanmar. sawvet@uvsyezin.edu.mm., Chel HM; Department of Pharmacology and Parasitology, University of Veterinary Science, Yezin, Nay Pyi Taw, 15013, Myanmar., Thu MJ; Department of Food and Drug Administration, Ministry of Health and Sports, Zabu Thiri, Nay Pyi Taw, 115011, Myanmar., Wai SS; Department of Veterinary Public Health, University of Veterinary Science, Yezin, Nay Pyi Taw, 15013, Myanmar., Eshita Y; Division of International Research Promotion, International Institute for Zoonosis Control, Hokkaido University, Sapporo, 001-0020, Japan., Nakao R; Laboratory of Parasitology, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan., Katakura K; Laboratory of Parasitology, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan., Htun LL; Department of Pharmacology and Parasitology, University of Veterinary Science, Yezin, Nay Pyi Taw, 15013, Myanmar.
Jazyk: angličtina
Zdroj: Acta parasitologica [Acta Parasitol] 2023 Dec; Vol. 68 (4), pp. 862-868. Date of Electronic Publication: 2023 Oct 13.
DOI: 10.1007/s11686-023-00721-x
Abstrakt: Purpose: Mosquitoes are important vectors that carry disease-causing agents that can affect humans and animals. DNA barcoding is a complementary identification which can be used to validate morphological characterization of mosquito species. The objectives of this study were to identify the mitochondrial sequence of the COI gene and to construct a molecular phylogeny based on the genetic divergence of the mosquito species studied.
Methods: In this study, DNA extraction and the amplification of the mitochondrial cytochrome oxidase subunit I genes (COI) were performed on pooled mosquito samples collected in Nay Pyi Taw area, Myanmar.
Results: Fragments of the COI gene showed 99-100% identity with sequences of Aedes aegypti, Armigeres subalbatus, Culex pipiens complex, and Cx. quinquefasciatus, respectively, deposited in GenBank. This study categorized two haplotypes from each Ar. subalbatus and Cx. pipiens complex COI gene sequence, as well as three haplotypes from Cx. quinquefasciatus COI gene sequences. The highest haplotype diversity and nucleotide diversity were observed in the Ar. subalbatus population (Hd = 1.0000; π = 0.0033), followed by the Cx. pipiens complex and Cx. quinquefasciatus populations.
Conclusion: This study provides useful information on the molecular identification and genetic diversity of mosquito vectors with medical and veterinary significance, which may assist in the improvement of mosquito control programs.
(© 2023. The Author(s) under exclusive licence to Witold Stefański Institute of Parasitology, Polish Academy of Sciences.)
Databáze: MEDLINE
Externí odkaz: