Identification of optimal conditions for human placental explant culture and extracellular vesicle release.

Autor:	Tekkatte C; Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Diego, La Jolla, CA 92037, USA.; Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037, USA., Lindsay SA; Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Diego, La Jolla, CA 92037, USA.; Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037, USA., Duggan E; Scintillon Institute, San Diego, CA 92121, USA.; Cellarcus Biosciences Inc, La Jolla, CA 92037, USA., Castro-Martínez A; Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Diego, La Jolla, CA 92037, USA.; Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037, USA., Hakim A; Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Diego, La Jolla, CA 92037, USA.; Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037, USA., Saldana I; Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Diego, La Jolla, CA 92037, USA.; Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037, USA., Zhang Y; Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Diego, La Jolla, CA 92037, USA.; Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037, USA.; Department of Obstetrics, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China., Zhou J; Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Diego, La Jolla, CA 92037, USA.; Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037, USA.; Department of Obstetrics, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China., Sebastian R; Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Diego, La Jolla, CA 92037, USA.; Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037, USA., Liu Y; Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Diego, La Jolla, CA 92037, USA.; Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037, USA.; Department of Obstetrics and Gynecology, SUN Yat-sen Memorial Hospital, SUN Yat-sen University, Guangzhou, Guangdong, China., Pontigon DS; Department of Pathology, University of California San Diego, La Jolla, CA 92037, USA., Meads M; Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037, USA.; Department of Pathology, University of California San Diego, La Jolla, CA 92037, USA., Liu TN; Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Diego, La Jolla, CA 92037, USA.; Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037, USA., Pizzo DP; Department of Pathology, University of California San Diego, La Jolla, CA 92037, USA., Nolan J; Scintillon Institute, San Diego, CA 92121, USA.; Cellarcus Biosciences Inc, La Jolla, CA 92037, USA., Parast MM; Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037, USA.; Department of Pathology, University of California San Diego, La Jolla, CA 92037, USA., Laurent LC; Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Diego, La Jolla, CA 92037, USA.; Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037, USA.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2023 Sep 26; Vol. 26 (10), pp. 108046. Date of Electronic Publication: 2023 Sep 26 (Print Publication: 2023).
DOI:	10.1016/j.isci.2023.108046
Abstrakt:	Extracellular vesicles (EVs) can mediate intercellular communication, including signaling between the placenta and maternal tissues. Human placental explant culture is a versatile in vitro model system to investigate placental function. We performed systematic studies in different tissue culture media types and oxygen tensions to identify a defined serum-free culture condition that supports high trophoblast viability and metabolism, as well as the release of similar populations of EVs, compared to traditional undefined conditions that contain media additives potentially contaminated with exogenous EVs. We also determined the time frame in which trophoblast viability and functionality remain optimal. Multiplex vesicle flow cytometry with classical EV and placenta-specific markers revealed three separate populations of explant-derived EVs: small CD63 + EVs; large PLAP+ EVs; and CD63-/PLAP- EVs. These culture and analytical approaches will enable in vitro modeling of short-term effects of environmental perturbations associated with pregnancy complications on placental function and EV release. Competing Interests: The authors declare no competing interests. (© 2023 The Authors.)
Databáze:	MEDLINE
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