A spectroscopy based prototype for the noninvasive detection of diabetes from human saliva using nanohybrids acting as nanozyme.

Autor: Roy L; Department of Applied Optics and Photonics, University of Calcutta, JD-2, Sector-III, Salt Lake, Kolkata, West Bengal, 700 106, India., Mondal S; Department of Chemical and Biological Sciences, S. N. Bose National Centre for Basic Sciences, Kolkata, 700106, India., Bhattacharyya N; Department of Radio Physics and Electronics, University of Calcutta, Kolkata, 700009, India., Ghosh R; Department of Chemical and Biological Sciences, S. N. Bose National Centre for Basic Sciences, Kolkata, 700106, India., Banerjee A; Department of Physics, Jadavpur University, 188, Raja Subodh Chandra Mallick Rd, Poddar Nagar, Jadavpur, Kolkata, West Bengal, 700032, India., Singh S; Department of Chemical and Biological Sciences, S. N. Bose National Centre for Basic Sciences, Kolkata, 700106, India.; Neo Care Inc, 27, Parker St, Dartmouth, NS, B2Y 2W1, Canada.; Electrical and Computer Engineering Department, Dalhousie University, 6299 South St, Halifax, NS, B3H 4R2, Canada., Chattopadhyay A; Department of Basic Science and Humanities, Techno International New Town Block, DG 1/1, Action Area 1 New Town, Rajarhat, Kolkata, 700156, India.; Department of Physics, Sister Nivedita University, DG 1/2 New Town, Action Area 1, Kolkata, 700156, India., Ahmed SA; Chemistry Department, Faculty of Applied Science, Umm Al-Qura University, 21955, Makkah, Saudi Arabia. skpal@bose.res.in.; Chemistry Department, Faculty of Science, Assiut University, Assiut, 71516, Egypt. skpal@bose.res.in., Jassas RS; Department of Chemistry, Jamoum University College, Umm Al-Qura University, 21955, Makkah, Saudi Arabia., Al-Rooqi MM; Chemistry Department, Faculty of Applied Science, Umm Al-Qura University, 21955, Makkah, Saudi Arabia., Moussa Z; Department of Chemistry, College of Science, United Arab Emirates University, P.O. Box 15551, Al Ain, Abu Dhabi, United Arab Emirates., Althagafi II; Chemistry Department, Faculty of Applied Science, Umm Al-Qura University, 21955, Makkah, Saudi Arabia., Bhattacharya D; Department of Gynecology & Obstetrics, Nil Ratan Sircar Medical College & Hospital, 138, AJC Bose Road, Sealdah, Raja Bazar, Kolkata, 700014, India., Bhattacharya K; Department of Applied Optics and Photonics, University of Calcutta, JD-2, Sector-III, Salt Lake, Kolkata, West Bengal, 700 106, India., Mallick AK; Department of Pediatrics, Nil Ratan Sircar Medical College and Hospital, Kolkata, 700014, India., Pal SK; Department of Chemical and Biological Sciences, S. N. Bose National Centre for Basic Sciences, Kolkata, 700106, India. saleh_63@hotmail.com.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Oct 12; Vol. 13 (1), pp. 17306. Date of Electronic Publication: 2023 Oct 12.
DOI: 10.1038/s41598-023-44011-y
Abstrakt: The recent prediction of diabetes to be a global pandemic invites a detection strategy preferably non-invasive, and bloodless to manage the disease and the associated complications. Here, we have synthesized chitosan polymer functionalized, organic-inorganic bio-compatible nano-hybrids of Mn 3 O 4 nanoparticles, and characterized it by utilizing several optical methodologies for the structural characterization which shows the Michaelis Menten (MM) kinetics for glucose and alpha-amylase protein (well-known diabetes biomarkers). We have also studied the potentiality for the detection of alpha-amylase in human salivary secretion which is reported to be strongly correlated with uncontrolled hyperglycemia. Finally, we have developed a prototype for the measurement of glucose (LOD of 0.38 mg/dL, LOQ of 1.15 mg/dL) and HbA1c (LOD of 0.15% and LOQ of 0.45%) utilizing the basic knowledge in the study for the detection of uncontrolled hyperglycemia at the point-of-care. With the limited number of clinical trials, we have explored the potential of our work in combating the diabetic pandemic across the globe in near future.
(© 2023. Springer Nature Limited.)
Databáze: MEDLINE
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