A pneumonectomy model to study flow-induced pulmonary hypertension and compensatory lung growth.

Autor:	Tsikis ST; Vascular Biology Program, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA; Department of Surgery, Boston Children's Hospital, Harvard Medical School, 300 Longwood Avenue, Fegan 3, Boston, MA 02115, USA., Klouda T; Division of Pulmonary Medicine, Boston Children's Hospital, Boston, MA 02115, USA., Hirsch TI; Vascular Biology Program, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA; Department of Surgery, Boston Children's Hospital, Harvard Medical School, 300 Longwood Avenue, Fegan 3, Boston, MA 02115, USA., Fligor SC; Vascular Biology Program, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA; Department of Surgery, Boston Children's Hospital, Harvard Medical School, 300 Longwood Avenue, Fegan 3, Boston, MA 02115, USA., Liu T; Division of Pulmonary Medicine, Boston Children's Hospital, Boston, MA 02115, USA., Kim Y; Division of Pulmonary Medicine, Boston Children's Hospital, Boston, MA 02115, USA., Pan A; Vascular Biology Program, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA; Department of Surgery, Boston Children's Hospital, Harvard Medical School, 300 Longwood Avenue, Fegan 3, Boston, MA 02115, USA., Quigley M; Vascular Biology Program, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA; Department of Surgery, Boston Children's Hospital, Harvard Medical School, 300 Longwood Avenue, Fegan 3, Boston, MA 02115, USA., Mitchell PD; Institutional Centers for Clinical and Translational Research, Boston Children's Hospital, Boston, MA 02115, USA., Puder M; Vascular Biology Program, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA; Department of Surgery, Boston Children's Hospital, Harvard Medical School, 300 Longwood Avenue, Fegan 3, Boston, MA 02115, USA. Electronic address: Mark.Puder@childrens.harvard.edu., Yuan K; Division of Pulmonary Medicine, Boston Children's Hospital, Boston, MA 02115, USA. Electronic address: Ke.Yuan@childrens.harvard.edu.
Jazyk:	angličtina
Zdroj:	Cell reports methods [Cell Rep Methods] 2023 Oct 23; Vol. 3 (10), pp. 100613. Date of Electronic Publication: 2023 Oct 11.
DOI:	10.1016/j.crmeth.2023.100613
Abstrakt:	In newborns, developmental disorders such as congenital diaphragmatic hernia (CDH) and specific types of congenital heart disease (CHD) can lead to defective alveolarization, pulmonary hypoplasia, and pulmonary arterial hypertension (PAH). Therapeutic options for these patients are limited, emphasizing the need for new animal models representative of disease conditions. In most adult mammals, compensatory lung growth (CLG) occurs after pneumonectomy; however, the underlying relationship between CLG and flow-induced pulmonary hypertension (PH) is not fully understood. We propose a murine model that involves the simultaneous removal of the left lung and right caval lobe (extended pneumonectomy), which results in reduced CLG and exacerbated reproducible PH. Extended pneumonectomy in mice is a promising animal model to study the cellular response and molecular mechanisms contributing to flow-induced PH, with the potential to identify new treatments for patients with CDH or PAH-CHD. Competing Interests: Declaration of interests The authors declare no competing interests relevant to this work. (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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