| Autor: |
Anggara K; Max-Planck Institute for Solid-State Research, DE-70569 Stuttgart, Germany., Sršan L; Institute of Organic Chemistry, University of Tübingen, DE-72076 Tübingen, Germany., Jaroentomeechai T; Copenhagen Center for Glycomics, Department of Cellular & Molecular Medicine, University of Copenhagen, DK-2200, Copenhagen, Denmark., Wu X; Max-Planck Institute for Solid-State Research, DE-70569 Stuttgart, Germany., Rauschenbach S; Max-Planck Institute for Solid-State Research, DE-70569 Stuttgart, Germany.; Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Oxford OX1 3TA, UK., Narimatsu Y; Copenhagen Center for Glycomics, Department of Cellular & Molecular Medicine, University of Copenhagen, DK-2200, Copenhagen, Denmark.; GlycoDisplay ApS, DK-2200, Copenhagen, Denmark., Clausen H; Copenhagen Center for Glycomics, Department of Cellular & Molecular Medicine, University of Copenhagen, DK-2200, Copenhagen, Denmark., Ziegler T; Institute of Organic Chemistry, University of Tübingen, DE-72076 Tübingen, Germany., Miller RL; Copenhagen Center for Glycomics, Department of Cellular & Molecular Medicine, University of Copenhagen, DK-2200, Copenhagen, Denmark., Kern K; Max-Planck Institute for Solid-State Research, DE-70569 Stuttgart, Germany.; Institut de Physique, École Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland. |
| Abstrakt: |
Proteins and lipids decorated with glycans are found throughout biological entities, playing roles in biological functions and dysfunctions. Current analytical strategies for these glycan-decorated biomolecules, termed glycoconjugates, rely on ensemble-averaged methods that do not provide a full view of positions and structures of glycans attached at individual sites in a given molecule, especially for glycoproteins. We show single-molecule analysis of glycoconjugates by direct imaging of individual glycoconjugate molecules using low-temperature scanning tunneling microscopy. Intact glycoconjugate ions from electrospray are soft-landed on a surface for their direct single-molecule imaging. The submolecular imaging resolution corroborated by quantum mechanical modeling unveils whole structures and attachment sites of glycans in glycopeptides, glycolipids, N-glycoproteins, and O-glycoproteins densely decorated with glycans. |
