Detecting DNA Loops Using Tethered Particle Motion.
| Autor: | Qian J; Department of Physics, Emory University, Atlanta, GA, USA., Collette D; Department of Physics, Emory University, Atlanta, GA, USA., Finzi L; Department of Physics, Emory University, Atlanta, GA, USA., Dunlap D; Department of Physics, Emory University, Atlanta, GA, USA. ddunlap@emory.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2694, pp. 451-466. |
| DOI: | 10.1007/978-1-0716-3377-9_21 |
| Abstrakt: | The range of motion of a micron-sized bead tethered by a single polymer provides a dynamic readout of the effective length of the polymer. The excursions of the bead may reflect the intrinsic flexibility and/or topology of the polymer as well as changes due to the action activity of ligands that bind the polymer. This is a simple yet powerful experimental approach to investigate such interactions between DNA and proteins as demonstrated by experiments with the lac repressor. This protein forms a stable, tetrameric oligomer with two binding sites and can produce a loop of DNA between recognition sites separated along the length of a DNA molecule. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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