Genetic structure and kdr mutations in Aedes aegypti populations along a road crossing the Amazon Forest in Amapá State, Brazil.

Autor: Souza BS; Laboratório de Biologia, Controle e Vigilância de Insetos Vetores (LBCVIV), Instituto Oswaldo Cruz (IOC)/FIOCRUZ, Rio de Janeiro, RJ, 21040-900, Brazil., Lima LF; Laboratório de Bioinformática, Instituto de Química (UFRJ), Rio de Janeiro, RJ, 21941-909, Brazil., Galardo AKR; Instituto de Pesquisas Científicas e Tecnológicas do Estado do Amapá/IEPA, Macapá, AP, 68.908-220, Brazil., Corbel V; Laboratório de Biologia, Controle e Vigilância de Insetos Vetores (LBCVIV), Instituto Oswaldo Cruz (IOC)/FIOCRUZ, Rio de Janeiro, RJ, 21040-900, Brazil.; Institut de Recherche Pour le Développement (IRD), MIVEGEC, CNRS, IRD, Université de Montpellier, 34090, Montpellier, France., Lima JBP; Laboratório de Biologia, Controle e Vigilância de Insetos Vetores (LBCVIV), Instituto Oswaldo Cruz (IOC)/FIOCRUZ, Rio de Janeiro, RJ, 21040-900, Brazil., Martins AJ; Laboratório de Biologia, Controle e Vigilância de Insetos Vetores (LBCVIV), Instituto Oswaldo Cruz (IOC)/FIOCRUZ, Rio de Janeiro, RJ, 21040-900, Brazil. ademirjr@ioc.fiocruz.br.; Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM), UFRJ, Rio de Janeiro, RJ, 21941-902, Brazil. ademirjr@ioc.fiocruz.br.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Oct 11; Vol. 13 (1), pp. 17167. Date of Electronic Publication: 2023 Oct 11.
DOI: 10.1038/s41598-023-44430-x
Abstrakt: Insecticide resistance in Aedes aegypti poses a significant threat to disease control. One form of resistance, caused by kdr mutations in the Na V gene, hinders vector control efforts in Brazil. Despite genetic differences typically accumulating among isolated populations, this mosquito can actively and passively disperse through human transportation. Our study investigated the genetic structure and spread of kdr mutations in Ae. aegypti populations across six localities in Amapá State, Brazil, within the Amazonian Forest. Using 12 microsatellite loci and qPCR methods, we assessed genetic structure and identified three common kdr mutations (V410L, V1016I, and F1534C). High prevalence of kdr alleles was observed in all localities, indicating widespread distribution in Amapá State. Microsatellite analysis revealed differentiation among mosquito populations, dividing them into two distinct clusters supported by Bayesian and DAPC analyses. Oiapoque, located along the northern border with French Guiana, exhibited the highest kdr frequencies and genetic differentiation compared to other localities. Our findings suggest genetic structure in Ae. aegypti populations in Amapá State, with some passive gene flow between clusters. The study underscores the need for continuous surveillance of Ae. aegypti populations to monitor the spread of insecticide resistance and inform effective vector control strategies.
(© 2023. Springer Nature Limited.)
Databáze: MEDLINE
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