Strain-level detection of Fusobacterium nucleatum in colorectal cancer specimens by targeting the CRISPR-Cas region.

Autor:	Shimomura Y; Dairy Science and Technology Institute, Kyodo Milk Industry Co. Ltd. , Tokyo, Japan., Sugi Y; Dairy Science and Technology Institute, Kyodo Milk Industry Co. Ltd. , Tokyo, Japan., Kume A; Dairy Science and Technology Institute, Kyodo Milk Industry Co. Ltd. , Tokyo, Japan., Tanaka W; Dairy Science and Technology Institute, Kyodo Milk Industry Co. Ltd. , Tokyo, Japan., Yoshihara T; Department of Gastroenterology and Hepatology, Yokohama City University School of Medicine , Yokohama, Japan., Matsuura T; Department of Gastroenterology and Hepatology, Yokohama City University School of Medicine , Yokohama, Japan., Komiya Y; Department of Gastroenterology and Hepatology, Yokohama City University School of Medicine , Yokohama, Japan., Ogata Y; Laboratory for Microbiome Sciences, RIKEN Center for Integrative Medical Sciences , Yokohama, Japan., Suda W; Laboratory for Microbiome Sciences, RIKEN Center for Integrative Medical Sciences , Yokohama, Japan., Hattori M; Laboratory for Microbiome Sciences, RIKEN Center for Integrative Medical Sciences , Yokohama, Japan., Higurashi T; Department of Gastroenterology and Hepatology, Yokohama City University School of Medicine , Yokohama, Japan., Nakajima A; Department of Gastroenterology and Hepatology, Yokohama City University School of Medicine , Yokohama, Japan., Matsumoto M; Dairy Science and Technology Institute, Kyodo Milk Industry Co. Ltd. , Tokyo, Japan.
Jazyk:	angličtina
Zdroj:	Microbiology spectrum [Microbiol Spectr] 2023 Dec 12; Vol. 11 (6), pp. e0512322. Date of Electronic Publication: 2023 Oct 11.
DOI:	10.1128/spectrum.05123-22
Abstrakt:	Importance: Fusobacterium nucleatum is one of the predominant oral bacteria in humans. However, this bacterium is enriched in colorectal cancer (CRC) tissues and may be involved in CRC development. Our previous research suggested that F. nucleatum is present in CRC tissues originating from the oral cavity using a traditional strain-typing method [arbitrarily primed polymerase chain reaction (AP-PCR)]. First, using whole-genome sequencing, this study confirmed an exemplary similarity between the oral and tumoral strains derived from each patient with CRC. Second, we successfully developed a method to genotype this bacterium at the strain level, targeting the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated system, which is hypervariable (defined as F. nucleatum -strain genotyping PCR). This method can identify F. nucleatum strains in cryopreserved samples and is significantly superior to traditional AP-PCR, which can only be performed on isolates. The new methods have great potential for application in etiological studies of F. nucleatum in CRC. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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