Histological and immunohistochemical studies of the fungiform and the circumvallate papillae through the life stages from 6- to 72-week-old Sprague-Dawley male rats.

Autor: Abdel-Maksoud FM; Department of Tissue and Developmental Biology, Osaka University Graduate School of Dentistry, Suita, Osaka, Japan.; Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt., Inui-Yamamoto C; Department of Tissue and Developmental Biology, Osaka University Graduate School of Dentistry, Suita, Osaka, Japan., Kawano A; Department of Tissue and Developmental Biology, Osaka University Graduate School of Dentistry, Suita, Osaka, Japan.; Department of Oral Health Sciences, Otemae College, Nishinomiya, Hyogo, Japan., Honma S; Department of Tissue and Developmental Biology, Osaka University Graduate School of Dentistry, Suita, Osaka, Japan., Saeki N; Department of Tissue and Developmental Biology, Osaka University Graduate School of Dentistry, Suita, Osaka, Japan.; Division of Special Care Dentistry, Osaka University Dental Hospital, Suita, Osaka, Japan., Abe M; Department of Tissue and Developmental Biology, Osaka University Graduate School of Dentistry, Suita, Osaka, Japan., Kuraki M; Department of Tissue and Developmental Biology, Osaka University Graduate School of Dentistry, Suita, Osaka, Japan., Ohba S; Department of Tissue and Developmental Biology, Osaka University Graduate School of Dentistry, Suita, Osaka, Japan., Wakisaka S; Department of Tissue and Developmental Biology, Osaka University Graduate School of Dentistry, Suita, Osaka, Japan.; Department of Dental Hygiene, Kansai Women's College, Kashiwara, Osaka, Japan.
Jazyk: angličtina
Zdroj: Anatomical record (Hoboken, N.J. : 2007) [Anat Rec (Hoboken)] 2024 Feb; Vol. 307 (2), pp. 414-425. Date of Electronic Publication: 2023 Oct 11.
DOI: 10.1002/ar.25338
Abstrakt: Taste sensitivity decreases with age. Therefore, we investigated the histological and immunohistochemical changes in the receptive fields circumvallate papilla (CvP) and fungiform papilla (FfP) to explore the mechanism underlying age-related changes in taste sensitivity in 6- to 72-week-old rats. We analyzed papilla size, the thickness of the keratin layer of the papilla and stratified squamous epithelium, taste bud size, the keratin layer around the taste pores in the CvP and FfP, and the number and distribution of taste buds in the CvP coronal section. We further assessed the expression of marker proteins for Type II and III cells, phospholipase C subtype beta 2 (PLCβ2), and synaptosomal-associated protein 25 (SNAP-25). The cellular activity of these taste cells was examined through co-localization with the senescence cell marker protein-30 (SMP30). There were no differences in the number of taste bud sections in the CvP among the age groups. However, the size of the CvP increased and the density of the taste bud area in the CvP area decreased with increasing age. In contrast, the number of cells with co-expression of SMP30, PLCβ2, and SNAP-25 decreased with age. Furthermore, the morphological structures of the CvP, FfP, and taste buds in these regions changed with age, but not the overall taste bud number in the CvP coronal section. The decrease in cell count with co-expression of SMP30 and PLCβ2, or SNAP-25 may indicate reduced cellular functions of taste cells with aging.
(© 2023 American Association for Anatomy.)
Databáze: MEDLINE
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