Methods used to achieve different levels of the neuronal differentiation process in SH-SY5Y and Neuro2a cell lines: An integrative review.

Autor: Dos Santos MG; Biomedical Science Postgraduate Program, State University of Ponta Grossa, Ponta Grossa, Paraná, Brazil., Gomes JR; Biomedical Science Postgraduate Program, State University of Ponta Grossa, Ponta Grossa, Paraná, Brazil., Costa MDM; Biomedical Science Postgraduate Program, State University of Ponta Grossa, Ponta Grossa, Paraná, Brazil.
Jazyk: angličtina
Zdroj: Cell biology international [Cell Biol Int] 2023 Dec; Vol. 47 (12), pp. 1883-1894. Date of Electronic Publication: 2023 Oct 10.
DOI: 10.1002/cbin.12093
Abstrakt: To study the process of neuronal differentiation, the human neuroblastoma (SH-SY5Y) and the murine neuroblastoma (Neuro2a) cell lines have proven to be effective models. For this approach, different protocols involving known neurotrophic factors and other molecules, such as retinoic acid (RA), have been assessed to better understand the neuronal differentiation process. Thus, the goal of this manuscript was to provide a brief overview of recent studies that have used protocols to promote neurodifferentiation in SH-SY5Y and Neuro2a cell lines and used acquired morphology and neuronal markers to validate whether differentiation was effective. The published results supply some guidance regarding the relationship between RA and neurotrophins for SH-SY5Y, as well a serum concentrations for both cell lines. Furthermore, they demonstrate the potential application of Neuro2a, which is critical for future research on neuronal differentiation.
(© 2023 International Federation of Cell Biology.)
Databáze: MEDLINE
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