Generating and Validating Transgenic Mosquitoes with Transposon-Mediated Transgenesis.
| Autor: | Bottino-Rojas V; Department of Microbiology and Molecular Genetics, University of California, Irvine, California 92697-4500, USA., James AA; Department of Microbiology and Molecular Genetics, University of California, Irvine, California 92697-4500, USA aajames@uci.edu.; Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697-3900, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Cold Spring Harbor protocols [Cold Spring Harb Protoc] 2024 Oct 01; Vol. 2024 (10), pp. pdb.prot108194. Date of Electronic Publication: 2024 Oct 01. |
| DOI: | 10.1101/pdb.prot108194 |
| Abstrakt: | Transposon-mediated transgenesis has revolutionized both basic and applied studies of mosquito vectors of disease. Currently, techniques such as enhancer traps and transposon tagging, which rely on remobilizable insertional mutagenesis, are only possible with transposon-based vector systems. Here, we provide general descriptions of methods and applications of transposon-based mosquito transgenesis. The exact procedures must be adapted to each mosquito species and comparisons of some differences among different mosquito species are outlined. A number of excellent publications showing detailed and specific protocols and methods are featured and referenced. (© 2024 Cold Spring Harbor Laboratory Press.) |
| Databáze: | MEDLINE |
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