Parallelized Acquisition of Orbitrap and Astral Analyzers Enables High-Throughput Quantitative Analysis.

Autor:	Stewart HI; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Grinfeld D; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Giannakopulos A; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Petzoldt J; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Shanley T; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Garland M; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Denisov E; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Peterson AC; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Damoc E; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Zeller M; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Arrey TN; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Pashkova A; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Renuse S; Thermo Fisher Scientific, 355 River Oaks Pkwy, San Jose, California 95134, United States., Hakimi A; Thermo Fisher Scientific, 355 River Oaks Pkwy, San Jose, California 95134, United States., Kühn A; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Biel M; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Kreutzmann A; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Hagedorn B; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Colonius I; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Schütz A; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Stefes A; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Dwivedi A; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Mourad D; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Hoek M; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Reitemeier B; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Cochems P, Kholomeev A; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Ostermann R; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Quiring G; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Ochmann M; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Möhring S; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Wagner A; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Petker A; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Kanngiesser S; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Wiedemeyer M; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Balschun W; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Hermanson D; Thermo Fisher Scientific, 355 River Oaks Pkwy, San Jose, California 95134, United States., Zabrouskov V; Thermo Fisher Scientific, 355 River Oaks Pkwy, San Jose, California 95134, United States., Makarov AA; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany., Hock C; Thermo Fisher Scientific, 11 Hannah-Kunath Str., 28199 Bremen, Germany.
Jazyk:	angličtina
Zdroj:	Analytical chemistry [Anal Chem] 2023 Oct 24; Vol. 95 (42), pp. 15656-15664. Date of Electronic Publication: 2023 Oct 10.
DOI:	10.1021/acs.analchem.3c02856
Abstrakt:	The growing trend toward high-throughput proteomics demands rapid liquid chromatography-mass spectrometry (LC-MS) cycles that limit the available time to gather the large numbers of MS/MS fragmentation spectra required for identification. Orbitrap analyzers scale performance with acquisition time and necessarily sacrifice sensitivity and resolving power to deliver higher acquisition rates. We developed a new mass spectrometer that combines a mass-resolving quadrupole, the Orbitrap, and the novel Asymmetric Track Lossless (Astral) analyzer. The new hybrid instrument enables faster acquisition of high-resolution accurate mass (HRAM) MS/MS spectra compared with state-of-the-art mass spectrometers. Accordingly, new proteomics methods were developed that leverage the strengths of each HRAM analyzer, whereby the Orbitrap analyzer performs full scans with a high dynamic range and resolution, synchronized with the Astral analyzer's acquisition of fast and sensitive HRAM MS/MS scans. Substantial improvements are demonstrated over previous methods using current state-of-the-art mass spectrometers.
Databáze:	MEDLINE
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